IL5 recombinant monoclonal antibody, clone 2E3
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- More Files
Flow Cytometry
Flow cytometric analysis of human peripheral blood leukocytes (PBL) with IL5 recombinant monoclonal antibody, clone 2E3 (Cat # RAB03587).
Human PBL cells were fixed using 2% PFA and permeabilized with 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control-black line) or the r version of RAB03587 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against human IL5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human IL5.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Neutralization
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of human peripheral blood leukocytes (PBL) with IL5 recombinant monoclonal antibody, clone 2E3 (Cat # RAB03587).
Human PBL cells were fixed using 2% PFA and permeabilized with 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control-black line) or the r version of RAB03587 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Neutralization
- Gene Info — IL5
Entrez GeneID
3567Gene Name
IL5
Gene Alias
EDF, IL-5, TRF
Gene Description
interleukin 5 (colony-stimulating factor, eosinophil)
Omim ID
147850Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq
Other Designations
B cell differentiation factor I|OTTHUMP00000065830|T-cell replacing factor|eosinophil differentiation factor|interleukin 5|interleukin-5
- Interactomes
- Pathways
- Diseases