Size : 10ml
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HyperChrom Q HP Agarose is a strong anion exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom Q HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (3) Fine particle size design, with high resolution.
HyperChrom Q HP Agarose chromatography media parameters
Name
Description
Chromatography media type
Strong anion exchange
Ligation
-N+(CH3)3 (quaternary ammonium).
Scaffolding
Highly cross-linked agarose
Average particle size
34 μm
Ion load
0.14-0.20 mmol Cl-/mL chromatography medium
Dynamic load
> 70 mg BSA/mL chromatography medium*
Flow rates are recommended
90-150 cm/h
Maximum flow rate
200 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability **
2-14
Solvent resistant
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents
Oxidant, anionic detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml BSA sample, BSA loading volume per unit volume (mL) (mg) when the penetration of BSA reaches 10%.
**After 7 days in the environment of chromatography at 40 °C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly.
30% and 75% are v/v, volume ratio.
Store the components at 4℃ for 5 years.
