GW280264X [866924-39-2]
Cat# HY-115670-25mg
Size : 25mg
Brand : MedChemExpress
GW280264X is the mixed ADAM10/TACE (ADAM17) metalloproteinases inhibitor. GW280264X potently blocks TACE (ADAM17) and ADAM10 with IC50s of 8.0 nM and 11.5 nM, respectively. ADAM10 and 17 modulate the immunogenicity of glioblastoma-initiating cells.
For research use only. We do not sell to patients.
GW280264X Chemical Structure
CAS No. : 866924-39-2
This product is a controlled substance and not for sale in your territory.
Based on 3 publication(s) in Google Scholar
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Description |
GW280264X is the mixed ADAM10/TACE (ADAM17) metalloproteinases inhibitor. GW280264X potently blocks TACE (ADAM17) and ADAM10 with IC50s of 8.0 nM and 11.5 nM, respectively[1]. ADAM10 and 17 modulate the immunogenicity of glioblastoma-initiating cells[2]. |
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IC50 & Target[1] |
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In Vitro |
The proliferation of GS-7 cells was significantly reduced upon treatment with GW280264X or ADAM10/17 co-knockdown[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assay[2]
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In Vivo |
Pharmacological inhibition of ADAM10 and ADAM17 improves functional recovery after spinal cord injury (SCI)[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Molecular Weight |
575.72 |
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Formula |
C28H41N5O6S |
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CAS No. |
866924-39-2 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=C(OCC1=CC=CC=C1)NCCCC[C@H](NC([C@H](CC(C)C)[C@@H](N(C=O)O)CCC)=O)C(NC2=NC=CS2)=O |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 125 mg/mL (217.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 98.28% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.7370 mL | 8.6848 mL | 17.3696 mL | 43.4239 mL |
5 mM | 0.3474 mL | 1.7370 mL | 3.4739 mL | 8.6848 mL | |
10 mM | 0.1737 mL | 0.8685 mL | 1.7370 mL | 4.3424 mL | |
15 mM | 0.1158 mL | 0.5790 mL | 1.1580 mL | 2.8949 mL | |
20 mM | 0.0868 mL | 0.4342 mL | 0.8685 mL | 2.1712 mL | |
25 mM | 0.0695 mL | 0.3474 mL | 0.6948 mL | 1.7370 mL | |
30 mM | 0.0579 mL | 0.2895 mL | 0.5790 mL | 1.4475 mL | |
40 mM | 0.0434 mL | 0.2171 mL | 0.4342 mL | 1.0856 mL | |
50 mM | 0.0347 mL | 0.1737 mL | 0.3474 mL | 0.8685 mL | |
60 mM | 0.0289 mL | 0.1447 mL | 0.2895 mL | 0.7237 mL | |
80 mM | 0.0217 mL | 0.1086 mL | 0.2171 mL | 0.5428 mL | |
100 mM | 0.0174 mL | 0.0868 mL | 0.1737 mL | 0.4342 mL |
GW280264X Related Classifications
- Metabolic Enzyme/Protease
- MMP