Goat anti-P114-RHO-GEF / ARHGEF18
Cat# EB06163
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB06163 | Goat Anti-P114-RHO-GEF / ARHGEF18 Antibody | Pep-ELISA, WB, IF | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18Official Symbol: ARHGEF18
Accession Number(s): NP_056133.2; NP_001124427.1
Human GeneID(s): 23370
Important Comments: This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
Immunogen
Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 36404918.
Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human
Specific References
This antibody has been successfully used in WB and IF on Human:
Reiko Satow, Yudai Suzuki, Shinobu Asada, Sae Ota, Masashi Idogawa, Shiori Kubota, Noi Ikeo, Atsuko Yoneda, Kiyoko Fukami
Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells.
iScience. 2022 Nov 4;25(12):105501.
PMID: 36404918
This antibody (previous batch) has been successfully used in WB on Medaka:
Herder C, Swiercz JM, Müller C, Peravali R, Quiring R, Offermanns S, Wittbrodt J, Loosli F.
ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation.
Development. 2013 Jul;140(13):2787-97.
PMID: 23698346
This antibody (previous batch) has been successfully used in WB on Human:
Terry SJ, Elbediwy A, Zihni C, Harris AR, Bailly M, Charras GT, Balda MS, Matter K.
Stimulation of Cortical Myosin Phosphorylation by p114RhoGEF Drives Cell Migration and Tumor Cell Invasion.
PLoS One. 2012;7(11):e50188.
PMID: 23185572
This antibody (previous batch) has been successfully used in WB, IF and IP on Human:
Terry SJ, Zihni C, Elbediwy A, Vitiello E, Leefa Chong San IV, Balda MS, Matter K.
Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis.
Nat Cell Biol. 2011 Feb;13(2):159-66.
PMID: 21258369
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol