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Glucose-6-phosphate (G6P) is an important intermediate for glucose to get into cells. G6P is involved in pentose phosphate pathway, glycolytic pathway or be stored as glycogen or starch. G6P is utilized by glucose 6-phosphate dehydrogenase to produce NAD(P)H, which is very important in red blood cells. The deficiency of G6PDH will lead to hemolytic anemia.
The Glucose-6-Phosphate Fluorometric Assay Kit provides a highly sensitive, simple, fast and convenient way for detection of G6P levels in various biological samples based on fluorometric method. In the assay, G6P is oxidized to generate a product which converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The kit can detect 10 to 500 pmoles G6P which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5 × during processing. The kit is more sensitive than the G6P Colorimetric Assay Kit for low G6P samples.
