Reagents and instruments for immunology, cell biology and molecular biology.
 
> COX4 Polyclonal Antibody

COX4 Polyclonal Antibody

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Cat# E-AB-40519-50

Size : 50uL

Brand : Elabscience

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Verified Samples Verified Samples in WB: Zebrafish
Dilution WB 1:2000-5000
Isotype IgG
Host Rabbit
Reactivity Zebrafish
Applications WB
Clonality Polyclonal
Immunogen Synthetic peptide derived from human COX41 protein conjugated to keyhole limpet haemocyanin.
Abbre COX4
Synonyms COX 4,   COX IV,   COX IV 1,   COX IV-1,   COX41,   COX4B,   COX4I1,   COX4I2,   COX4L2,   COXIV,   Cox4,   Cox4a,   Cy,   Cytochrome c oxidase polypeptide IV,   Cytochrome c oxidase subunit 4 isoform 1,   Cytochrome c oxidase subunit 4 isoform 1 mitochondrial,   mitochondrial,  AL024441
Swissprot
Calculated MW 17 kDa
Observed MW 17 kD
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Membrane, Mitochondrion, Mitochondrion inner membrane.
Concentration 2 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer,  Metabolism,  Signal Transduction,  Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical g
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Unconjugated

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