Reagents and instruments for immunology, cell biology and molecular biology.

CD8 alpha, beta (CD8 alpha, CD8a, CD8 alpha Chain, CD8 Antigen alpha Chain, CD8 Antigen alpha Polypeptide (p32), CD8 beta, CD8b, CD8 Antigen beta Polypeptide 1, CD8B1, Leu2 T Lymphocyte Antigen, Leu-2, Leu2, Ly3, LYT3, MAL, MGC119115, OKT8 T cell Antigen, p32, T cell Antigen Leu2, T cell Co-receptor, T cell Surface Glycoprotein CD8 alpha Chain, T cell Surface Glycoprotein CD8 beta Chain, T Lymphocyte Differentiation Antigen T8/Leu-2, T8 T cell Antigen) (FITC)

Print

Cat# C2259-99Y-FITC-100ul

Size : 100ul

Brand : US Biological

Request more information

C2259-99Y-FITC CD8 alpha, beta (CD8 alpha, CD8a, CD8 alpha Chain, CD8 Antigen alpha Chain, CD8 Antigen alpha Polypeptide (p32), CD8 beta, CD8b, CD8 Antigen beta Polypeptide 1, CD8B1, Leu2 T Lymphocyte Antigen, Leu-2, Leu2, Ly3, LYT3, MAL, MGC119115, OKT8 T cell Antigen, p32, T cell Antigen Leu2, T cell Co-receptor, T cell Surface Glycoprotein CD8 alpha Chain, T cell Surface Glycoprotein CD8 beta Chain, T Lymphocyte Differentiation Antigen T8/Leu-2, T8 T cell Antigen) (FITC)

Clone Type

Polyclonal

Host

mouse

Source

feline

Swiss Prot

P41688

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IHC

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:|Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. ||Recommended Dilution:|Flow Cytometry: 10ul labels 10e6 cells in 100ul|Immunohistochemistry: frozen|Optimal dilutions to be determined by the researcher. ||Hybridoma: |NS0 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: vpg9|Host: mouse|Source: feline|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Feline thymocytes.|Specificity: Recognizes the feline homolog of human CD8. Specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (3). Does not react with human.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Feline thymocytes.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the feline homolog of human CD8. Specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (3). Does not react with human.

References

1. Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system. P 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency. Oxford University Press, Oxford. 2. Callanan, J.J., Racz, P., Thompson, H. and Jarrett, O., (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS. In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. P. 115-136. 3. Shimojima, M. et al. (1998) Characterization of anti-feline CD8 monoclonal antibodies. Vet.Immunol. Immunopathol. 61: 17-23.