CD40 Human, IgG2a Fc Mouse, Fusion Protein

Cat# C2392-02K-25ug

Size : 25ug

Brand : US Biological

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C2392-02K CD40 Human, IgG2a Fc Mouse, Fusion Protein

Clone Type
Polyclonal
Grade
Affinity Purified
Applications
E FC
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching. CD40 Human, IgG2a Fc Mouse, Fusion Protein is recognized by anti-human CD40 antibody and binds to human CD154. This binding can be blocked by anti-CD154 antibody.||Molecular Structure: |A soluble fusion protein consisting of the extracellular (173aa) domain of human CD40 fused to murine IgG2a Fc (233aa).||Applications: |Suitable for use in ELISA and Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 80ul at 5ug/ml labels 5x10e5 Jurkat-4G cells|Optimal dilutions to be determined by the researcher.||Flow Cytometry Protocol:|1. Jurkat-4G (a CD154 positive subclone of Jurkat)cells were stimulated 6 hours in the presence of PMA and Ionomycin (to increase CD154 expression). |2. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of C2392-02K at a concentration of  5ug/ml. |3. Cells were washed twice and incubated with 50ul secondary reagent IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. |4. Cells stained positive with a mean shift of 0.22 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: CHO cells|Purity: Purified by affinity chromatography. |Concentration: ~0.5mg/ml|Form: Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate
Purity
Purified by affinity chromatography.
References
1. T.A. Foy, et al, (1996) Annu Rev Immunol 14: 591-617. 2. M.E. Ozaki, et al, (1997) J Immunol 159: 214-221.