Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) (Not for Export EU)

Cat# C2087-22J-200ul

Size : 200ul

Brand : US Biological

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C2087-22J Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) (Not for Export EU)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
P42574
Isotype
IgG
Grade
Serum
Applications
WB
Crossreactivity
Bo Ca Ch Gp Hm Hu Mk Mo Po Rb Rt Sh Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or ?executioner? caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.||Applications: |Suitable for use Western Blot. Other applications not tested.||Recommended Dilutions:|Western Blot (ECL): 1:5000 detects a band at ~36kD|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: Not Determined|Form: Supplied as a liquid.|Purity: Serum |Immunogen: Synthetic peptide corresponding to a portion of human Caspase-3. The sequence differs from rat capase-3 by 1aa and from mouse capase-3 by 2aa.|Specificity: Recognizes human Caspase-3. Species Crossreactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to a portion of human Caspase-3. The sequence differs from rat capase-3 by 1aa and from mouse capase-3 by 2aa.
Form
Supplied as a liquid.
Purity
Serum
Specificity
Recognizes human Caspase-3. Species Crossreactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.
References
1. GenBank Accession #U26943 2. Zhivotovsky, B., Samali, A., Gahm, A and Orrenius, S. (1999) Cell Death Differ. 6: 644-651. 3. Cryns, V. and Yuan, J. (1998) Genes Dev. 12: 1551-1570. 4. Thornberry, N.A. and Lazebnik, Y. (1998) Science 281: 1312-1322. 5. Erhart, P and Cooper, G.M. (1996) J. Biol. Chem 271: 17601-17604 6. Nicholson, D.W. and Thornberry, N.A. (1997) Trends Biochem. Sci. 22: 299-306. 7. Hundal, R.S., Salh, B.S., Schrader, J.W., Gomez-Munoz, A., Duronio, V. and Steinbrecher, U.P. (2001) J. of Lipid Research. 42: 1483-1491. 8. Raina, A.K., Hochman, A., Zhu, X., Rottkamp, C.A., Nunomura, A., Siedlak, S.L., Boux, H., Castellani, R.J., Perry, G. and Smith, M.A. (2001) Acta. Neuropathol. 101(4): 305-310. 9. Gomez-Munoz, A., Kong, J., Salh, B. and Steinbrecher, U.P. (2003) FEBS Lett. 539(1-3):56-60.