BPTF/FALZ TR-FRET, BioAssay™ Kit
Cat# 298337-1Kit
Size : 1Kit
Brand : US Biological
298337 BPTF/FALZ TR-FRET, BioAssay™ Kit
Clone Type
PolyclonalSwiss Prot
Q12830Shipping Temp
Blue IceStorage Temp
-20°C/-70°CKit comes in a convenient format with purified BPTF/FALZ, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.||The BPTF TR-FRET BioAssay™ Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.||Applications: |Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.||Kit Components:|White Nonbinding low volume microtiter plate (Corning), 1x plate|BPTF(FALZ), GST-tag, 1x10ug|BET Bromodomain Ligand, 1x75ul |Non-acetylated Ligand 1, 1x15ul|Tb donor, 1x20ul |Dye-labeled acceptor, 1x20ul |3x BPTF TR-FRET Assay Buffer, 1x4ml ||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.