Anti-USH2A antibody (150-180)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.51 µg/µl
Dilution Range:	WB: 1:500 DB: 1:4, 000 ELISA: 1:4, 000 IP: 1:200 IHC: 1:250 ICC: 1:250 IF: 1:250 CFM: 1:250
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	USH2A
Gene ID:	7399
Uniprot ID:	USH2A_HUMAN
Immunogen Region:	150-180
Specificity:	This antibody will detect both Usherin isoform A and Usherin isoform B.
Immunogen:	Synthetic peptide taken within amino acid region 150-180 on Usherin syndrome 2A protein.

Tissue Specificity	Present in the basement membrane of many, but not all tissues. Expressed in retina, cochlea, small and large intestine, pancreas, bladder, prostate, esophagus, trachea, thymus, salivary glands, placenta, ovary, fallopian tube, uterus and testis. Absent in many other tissues such as heart, lung, liver, kidney and brain. In the retina, it is present in the basement membranes in the Bruch's layer choroid capillary basement membranes, where it localizes just beneath the retinal pigment epithelial cells (at protein level). Weakly expressed. Isoform 2 is expressed in fetal eye, cochlea and heart, and at very low level in brain, CNS, intestine, skeleton, tongue, kidney and lung. Isoform 2 is not expressed in stomach and liver. In adult tissues, isoform 2 is expressed in neural retina and testis, and at low level in brain, heart, kidney and liver. Isoform 1 displays a similar pattern of expression but is expressed at very low level in fetal cochlea.
Function	Involved in hearing and vision as member of the USH2 complex. In the inner ear, required for the maintenance of the hair bundle ankle formation, which connects growing stereocilia in developing cochlear hair cells. In retina photoreceptors, the USH2 complex is required for the maintenance of periciliary membrane complex that seems to play a role in regulating intracellular protein transport.
Protein Name	Usherin Usher Syndrome Type Iia Protein Usher Syndrome Type-2a Protein
Cellular Localisation	Cell Projection Stereocilium Membrane Single-Pass Type I Membrane Protein Component Of The Interstereocilia Ankle Links In The Inner Ear Sensory Cells In Photoreceptors Localizes At A Plasma Membrane Microdomain In The Apical Inner Segment That Surrounds The Connecting Cilia Called Periciliary Membrane Complex Isoform 2: Secreted
Alternative Antibody Names	Anti-Usherin antibody Anti-Usher Syndrome Type Iia Protein antibody Anti-Usher Syndrome Type-2a Protein antibody Anti-USH2A antibody

Information sourced from Uniprot.org

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