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Acetate is an important molecule for biosynthesis. In living organisms, acetate is used to generate acetyl coenzyme A (acetyl-CoA), which is central to metabolism of fats and carbohydrates. Acetyl-CoA is also involved in the synthesis of neurotransmitter acetylcholine. Acetylation/deacetylation of proteins is important for post-translational regulation of their functions. The Acetate Colorimetric Assay Kit provides a sensitive, simple and fast way for detection of acetate levels in various samples based on colorimetric method. In the assay, in the presence of Acetate Enzyme Mix and Acetate Substrate Mix, acetate is converted to an intermediate, which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The kit can detect less than 20 µM acetate in various samples.
