ABTS (diammonium salt) [30931-67-0]
Cat# HY-15902-1g
Size : 1g
Brand : MedChemExpress
ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
For research use only. We do not sell to patients.
ABTS diammonium salt Chemical Structure
CAS No. : 30931-67-0
This product is a controlled substance and not for sale in your territory.
Based on 3 publication(s) in Google Scholar
Description |
ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate. |
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In Vitro |
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H2O2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H2O2. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 μM[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
548.68 |
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Formula |
C18H24N6O6S4 |
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CAS No. |
30931-67-0 |
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Appearance |
Solid |
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Color |
Light green to green |
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SMILES |
CCN1C(C=CC(S(=O)(O)=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)(O)=O)=C3)\N4CC.N.N |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light) |
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Solvent & Solubility |
In Vitro:
H2O : ≥ 50 mg/mL (91.13 mM) DMSO : 20.83 mg/mL (37.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation |
Purity: 99.86% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O | 1 mM | 1.8226 mL | 9.1128 mL | 18.2256 mL | 45.5639 mL |
5 mM | 0.3645 mL | 1.8226 mL | 3.6451 mL | 9.1128 mL | |
10 mM | 0.1823 mL | 0.9113 mL | 1.8226 mL | 4.5564 mL | |
15 mM | 0.1215 mL | 0.6075 mL | 1.2150 mL | 3.0376 mL | |
20 mM | 0.0911 mL | 0.4556 mL | 0.9113 mL | 2.2782 mL | |
25 mM | 0.0729 mL | 0.3645 mL | 0.7290 mL | 1.8226 mL | |
30 mM | 0.0608 mL | 0.3038 mL | 0.6075 mL | 1.5188 mL | |
H2O | 40 mM | 0.0456 mL | 0.2278 mL | 0.4556 mL | 1.1391 mL |
50 mM | 0.0365 mL | 0.1823 mL | 0.3645 mL | 0.9113 mL | |
60 mM | 0.0304 mL | 0.1519 mL | 0.3038 mL | 0.7594 mL | |
80 mM | 0.0228 mL | 0.1139 mL | 0.2278 mL | 0.5695 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ABTS diammonium salt Related Classifications
- Others
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
ABTS diammonium30931-67-0AzBTS-(NH4)2Fluorescent DyeInhibitorinhibitorinhibit