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> 3-Methyladenine [5142-23-4]

3-Methyladenine [5142-23-4]

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Cat# HY-19312-50mg

Size : 50mg

Brand : MedChemExpress

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Description

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[1].

IC50 & Target[1]

PtdIns3Kγ

60 μM (IC50, Cell Assay)

Vps34

25 μM (IC50, Cell Assay)

Autophagy

 

Mitophagy

 

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
MIA PaCa-2 EC50
> 100 μM
Compound: 3 mA
Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
[PMID: 28105281]
In Vitro

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:
The recommended concentration of 3-MA is approximately 0.5-10 mM in cell culture. DMSO stock solution is not recommended. We suggest that you can weigh out the amount of 3-MA you required before your experiment. Then please dissolve it in medium and sterilize with a 0.22 μm filter.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Methyladenine Related Antibodies

Cell Viability Assay[2]

Cell Line: HeLa cells
Concentration: 0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time: 0 hour, 24 hours and 48 hours
Result: Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

Cell Autophagy Assay[2]

Cell Line: HeLa cells
Concentration: 5 mM
Incubation Time: 24 hours
Result: Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

Western Blot Analysis[2]

Cell Line: HeLa cells
Concentration: 5 mM
Incubation Time: 0 hour, 12 hours, 24 hours and 48 hours
Result: Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.
In Vivo

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)[3]
Dosage: 1.5 mg/100 g (1000 μM)
Administration: Intraperitoneal injection
Result: Alleviated Sodium Taurocholate-Induced SAP.
Molecular Weight

149.15

Formula

C6H7N5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C2N=CN=C2N(C)C=N1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (33.52 mM; Need ultrasonic)

DMSO : 8.33 mg/mL (55.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7047 mL 33.5233 mL 67.0466 mL
5 mM 1.3409 mL 6.7047 mL 13.4093 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 25 mg/mL (167.62 mM); Clear solution; Need ultrasonic and warming and heat to 50°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

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