Pfu polymerases
Pfu DNA polymerase has superior thermostability and proofreading properties compared to other thermostable polymerase
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kDa. It can amplify DNA target up to 2 kb (simple template). The elongation velocity is 0.2~0.4 kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.
Features
- 3'-5' exonuclease activity provides a low error rate
- One of the most thermostable DNA polymerases known
- Lack of extendase activity means no unwanted 3' overhangs
- Optimal for blunt-ended PCR cloning
- Optimum temperature near 75°C
- 95% active after 1hour incubation at 98°C
Applications
- High-fidelity PCR and primer-extension reactions
- High fidelity PCR for cloning into blunt-ended vectors
- Site-directed mutagenesis
