Flow cytometry primary antibodies

Flow cytometry primary antibodies

Primary antibodies are vital in flow cytometry, enabling precise detection and analysis of specific antigens on cells. Their significance in research can be highlighted through the following properties:

  • Target Specificity: Primary antibodies uniquely bind to specific epitopes, allowing for accurate identification of cell populations based on surface markers. This specificity is crucial for distinguishing between similar cell types, enhancing the reliability of results in complex biological systems.
  • Single-Cell Analysis: Flow cytometry facilitates the examination of individual cells, crucial for understanding cellular heterogeneity and identifying rare cell populations within complex samples. This capability is particularly valuable in cancer research, where identifying tumor subpopulations can inform treatment strategies.
  • Enhanced Data Quality: The careful selection of validated primary antibodies is critical for generating reliable data, as they help distinguish specific from nonspecific binding, thereby improving experimental accuracy. High-quality antibodies reduce background noise, leading to clearer and more interpretable results.

The ability to conduct multi-parameter analyses using panels of primary antibodies further enhances flow cytometry's utility, allowing researchers to explore complex biological questions with greater depth and precision.

Primary antibodies are extensively used across various fields, including immunology—characterizing immune cell subsets; oncology—monitoring tumor responses; and infectious disease research—studying pathogen interactions.

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  • Unconjugated 295099
  • PE 24681
  • FITC 21716
  • APC 21529
  • Biotin 16718
  • HRP 11987
  • Cy3 9523
  • PerCp 9195
  • Cy7 7494
  • iFluor™647 7010
  • Cy5 5735
  • Cy5.5 5527
  • RBITC 4046
  • PE/Cy7 3579
  • PE/Cy5 3305
  • PerCP/Cy5.5 3247
  • PE/Cy5.5 2927
  • PE-Cy5 2538
  • PE-Cy7 2417
  • PerCP-Cy5.5 2385
  • PE-Cy5.5 2262
  • APC-CY5.5 2228
  • RPE 2196
  • APC/Cy7 2079
  • APC-Cy7 1987
  • APC/Cy5.5 1880
  • Alexa Fluor 488 1688
  • Alexa Fluor 555 1632
  • Alexa Fluor 350 1631
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  • azide free 895
  • low endotoxin 889
  • Alexa Fluor 647 701
  • MaxLight 490 535
  • MaxLight 650 532
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  • AbFluor™ 488 503
  • Pacific Blue 332
  • Low Endotoxin/Azide Free 290
  • Red 286
  • Texas Red® 234
  • Alexa Fluor 680 164
  • Alexa Fluor 568 161
  • Alexa Fluor 594 161
  • CF®488 92
  • DyLight® 488 78
  • DyLight® 550 69
  • violetFluor 450 51
  • Alexa Fluor 700 40
  • SPRD 39
  • PerCP-Cyanine5.5 38
  • purified 36
  • redFluor 710 34
  • R-PE 32
  • CF®488A 24
  • FITC/PE 24
  • CF®405M 23
  • TRITC 23
  • CF®568 20
  • mfluor™450 20
  • APC-C750 14
  • iFluor™488 13
  • MaxLight 405 12
  • MaxLight 750 12
  • mFluor™540 12
  • Atto 390 10
  • Atto 488 10
  • Atto 594 10
  • AP 8
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  • PE/Texas Red 7
  • CF®405L 6
  • CF®405S 6
  • CF®514 6
  • CF®660C 6
  • PE-Cy3 6
  • DyLight 488 5
  • PE-Texas Red 5
  • ALP 4
  • DY-634 4
  • FITC/PE/PE-Cy5 4
  • PE-DL594 4
  • Rhodamine 4
  • Alkaline Phosphatase 3
  • Allophycocyanin 3
  • DyLight 550 3
  • DyLight 650 3
  • FITC/PE/PerCP-Cy5.5 3
  • Gold 3
  • R-Phycoerythrin 3
  • iFluor™594 3
  • CF®405 2
  • CF®555 2
  • Magnetic beads 2
  • AbFluor™ 594 1
  • Alexa Fluor 405 1
  • Atto 647N 1
  • BSA 1
  • Beads 1
  • DTAF 1
  • human 420802
  • mouse 229356
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  • zebrafish 1317
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  • all species 1014
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  • xenopus 502
  • drosophila 241
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  • bacteria 144
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  • frog 83
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  • pigeon 21
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  • duck 16
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  • bird 12
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  • camel 5
  • sars-cov 1
  • rabbit 268576
  • mouse 175320
  • rat 34011
  • human 10348
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  • guinea pig 337
  • e. coli 141
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  • virus 28
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  • pig 6
  • c. elegans 4
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  • prim 1
  • Primary antibody 517821
  • FC
  • WB 289976
  • IHC 167705
  • IF 164601
  • ELISA 126753
  • ICC 101662
  • IP 34444
  • FA 6598
  • Assay 2627
  • Blocking 2195
  • FLISA 934
  • Cell depletion 698
  • Neutralization 586
  • Staining 562
  • ChIP 438
  • Microscopy 290
  • Inhibition assay 202
  • Radioimmunoassay 195
  • Cellular activation 194
  • Dot blot 162
  • Stimulation 108
  • Cell separation 92
  • Mass cytometry 82
  • in-vivo 78
  • ELISPOT 68
  • Immunolocalization 48
  • Immunoassay 45
  • Agglutination 31
  • Co-stimulation 31
  • Affinity binding 25
  • Multiplex assay 23
  • Purification 21
  • Immunodiffusion 17
  • ISH 12
  • in-vitro 10
  • Detection 8
  • Proximity Ligation Assay 8
  • EMSA 4
  • Chemiluminescence 3
  • Immunoradiometric assay 3
  • Lateral Flow 3
  • Chromatography 2
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  • Immunostaining 1
  • array 1
  • Polyclonal 217489
  • Monoclonal 42226
  • Recombinant 11658
  • M1/70 591
  • GK1.5 566
  • 145-2C11 525
  • 53-6.7 517
  • RB6-8C5 504
  • 1D3 502
  • N418 481
  • PK136 443
  • 1A8 423
  • 2.4G2 423
  • 17A2 418
  • IM7 394
  • CI:A3-1 370
  • PC-61.5.3 365
  • 30-F11 363
  • DX5 363
  • M5/114 354
  • 3G8 351
  • RA3.3A 1/6.1 341
  • D7 334
  • polyclonal 328
  • 390 327
  • L243 326
  • GL-1 322
  • RPA-T4 320
  • 5H4 319
  • TER-119 316
  • Monts 1 314
  • 10.1 309
  • 2B8 304
  • OX-38 301
  • 16-10A1 299
  • CXCR3-173 299
  • UCHL1 298
  • UCHT1 297
  • YN1/1.7.4 296
  • HIT2 295
  • Mel14 293
  • 53-7.3 292
  • CD28.2 291
  • 2H7 289
  • M1/69 289
  • 30H12 288
  • FD441.8 287
  • M/K-2.7 285
  • DREG56 284
  • HM48-1 281
  • R17 217.1.3/TIB-219 281
  • 29F.1A12 280
  • MIAP410 280
  • OKT3 279
  • H57-597 (HB218) 277
  • 104.2 276
  • 4RA10 276
  • BU63 276
  • HI30 276
  • HI100 274
  • FGK4.5/FGK45 272
  • W6/32 272
  • M1/42 270
  • 37.51 269
  • A7R34 267
  • Cy34.1 265
  • 01 262
  • 19G11 257
  • UCHT2 257
  • 10F.9G2 248
  • Sa14-2 246
  • ICRF44 245
  • M290 240
  • FN50 231
  • OKT-8 231
  • HI98 229
  • HIP8 229
  • OX-1 228
  • 05 221
  • 6H6 221
  • TS2/16.2.1 221
  • E11 220
  • 6C5 217
  • 03 211
  • C5 211
  • 15.2 201
  • HIM6 201
  • CC2C6D4 200
  • AFS98 198
  • CB-CALLA 198
  • RA3-6B2 197
  • G28.5 196
  • LN2 195
  • OX-8 195
  • A019D5 192
  • H1.2F3 192
  • A20 191
  • CB19 191
  • HNK-1 189
  • O323 189
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