Classical DNA polymerases
Taq DNA polymerase is a thermostable DNA polymerase isolated from Thermus aquaticus.
This enzyme has a molecular weight of 95 kDa and exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct t-A cloning.Taq DNA Polymerase lacks 3'-5' exonuclease activity and proofreading ability.
Taq DNA polymerase can withstand prolonged incubation at high temperatures without a significant loss of activity and is widely used in assays for molecular biology, like routine PCR and cloning PCR.
We offer a wide range of Taq DNA polymerase for use in PCR. Please find below a selection of Taq DNA polymerase with main features :
| Name | Taq DNA polymerase | EsayTaq |
| Product code | NB-03-0083 to 0087 | AP111 |
| Type | Taq polymerase | Taq polymerase |
| Speed | 0.9-1.2 kb/min | 1-2 kb/min |
| Amplicon size | < 5kb | < 4kb |
| 5'-3' exonuclease activity | Yes | Yes |
| 3'-5' exonuclease activity | No | No |
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