Zn SepFast MAG
Cat# 190104
Size : 5ml
Brand : BioToolomics
Introduction
Magnetic IMAC (immobilised metal affinity chromatography) resin is typically useful in purifying small quantity low concentration histidine-tagged proteins. For example, when 10 – 50 µl of resin is required for protein purification, it is difficult to handle such small quantities in columns. Magnetic IMAC resins can be easily processed with the aid of a magnet. The liquid / solid separation required in each purification step (e.g. equilibration, binding, washing and elution) is readily achieved with the aid of a magnet (e.g. magnetic stirring bar or other commercial magnetic devices).
Key benefits:
- Tailored base matrix design for batch magnetic purification of proteins
- High binding capacity (could be over 40 mg/ml)
- Simple and convenient operation, no chromatography training required
- Flexible operational conditions
- Low cost
- A choice of three types of immobilised metal ions available
- Very low metal leakage
- Full operation manual (including purification conditions) supplied
Product Characteristics
Particle size | 50 – 150 µm |
Base matrix | Cross-linked 6% agarose encapsulating magnetic particles |
Metal ion capacity | Approx. 12 - 25 µmol / ml resin |
Protein binding capacity | Depends on the type of proteins and binding conditions; could be over 40 mg/ml resin* |
Chemical stability** | Stable in0.01M HCl and 1% SDS tested for 30 mins;0.5 M NaOH tested for overnight; |
pH stability** | 2-14 (<2 h)4-12 (up to one week) |
Storage | 20% ethanol at 4oC |
*Tested with nickel ion charged; **Tested with the absence of metal ion