Tubulin beta

TUBB3 encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Tubulin beta-3 chain is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.||Applications: |Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA. Other applications not tested.||Recommended Dilution:|Western Blot: 1:500-1:2000|Immunofluorescence: 1:200-1:1000|Immunohistochemistry (paraffin): 1:100-1:300|ELISA: 1:5000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.