TransScript® First-Strand cDNA Synthesis SuperMix
Cat# AT301-02
Size : 50rxns×20ulsystem
Brand : TransGen Biotech
ManualThe product uses RNA as a template to efficiently synthesize the first-strand cDNA by TransScript® RT/RI Enzyme Mix and
2×TS Reaction Mix. It is easy to operate and can reduce contamination risk.
Features
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.
• cDNA up to 12 kb.
Applications
Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AT301-02 | AT301-03 |
| TransScript® RT/RI Enzyme Mix | 50 μl | 100 μl |
| 2×TS Reaction Mix | 500 μl | 1 ml |
| Random Primer (N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo (dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
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