TransNGS® ATAC-Seq Library Prep Kit for Illumina®

Cat# KP171-01

Size : 12rxns

Brand : TransGen Biotech

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TransNGS® ATAC-Seq Library Prep Kit for Illumina®

Catalog Number: KP171-01

Price:Please inquire first

Specification:
12 rxns
96 rxns

Product Details

TransNGS® ATAC-Seq Library Prep Kit for Illumina® is developed for the Illumina high-throughput  sequencing platform and is suitable for constructing ATAC libraries from mammalian cells. The resulting  libraries can be used for Single-Read or Paired-End sequencing. This kit utilizes a novel transposase enzyme  technology for library construction targeting chromatin open regions, with experiments completed in only 3  hours. It requires minimal cell input, providing high-resolution sequencing profiles with starting cell amounts  ranging from 50 to 50,000 cells.

Features

· Time saving and simple operation. 

· Minimal cell input requirement. Library construction can be completed with as few as 50 cells.

Applications

Preparing chromatin open region fragments into short fragment libraries suitable for the Illumina high-throughput sequencing platform.

Storage

at -20℃ for one year.

Shipping

Dry ice (-70℃)

Product Composition

Component

KP171-01(12 rxns) KP171-02(96 rxns) 
Cell Lysis Buffer 24 μl        192 μl 
Lysis Enhancer 12 μl96 μl  
4×Insertion Buffer 90 μl720 μl 
Insertion Enhancer 1 12 μl96 μl
Insertion Enhancer 2 12 μl96 μl 
Tn5-50 Enzyme Mix 48 μl384 μl 
4×Tn5 Digestion Buffer 120 μl960 μl
TransNGS ATAC-Seq Library Amplification SuperMix I(2×) 300 μl4×600 μl 
TransNGS ATAC-Seq Library Amplification SuperMix II(2×) 300 μl 4×600 μl 
Library Elution Buffer600 μl 5 ml 
Nuclease-free Water1 ml 
2 × 5 ml 
Tn5 Storage Buffer600 μl5 ml