Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | TIBC |
Sample type | Serum |
Sensitivity | 0.03 mg/L |
Detection range | 0.03-50 mg/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 50 min |
Precision | Average inter-assay CV: 4.700%Average intra-assay CV: 3.400% |
Other instruments required | Micropipettor, Water bath, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl) |
Storage | 2-8℃ |
Valid period | 12 months |
Images
TIBC was significantly changed after treated.
R M Adel et al investigate the destructive effect of iron overload by silver nanocomposite. Total iron binding capacity (TIBC) in rat serum was determined using TIBC colorimetric assay kit (E-BC-K071-S).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-50 mg/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Rat serum | 1 |
Porcine serum | 1 |
Rabbit serum | 1 |
Chicken serum | 1 |
Cynomolgus monkey serum | 1 |
Note: The diluent is double distilled water or normal saline (0.9% NaCl) .
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849
Destructive effect of iron overload in brain tissue of albino rats: Ameliorative role of silver immobilized organo-modified casein nanocomposite as co-treating agent with DeferasiroxPMID: 34052583
Sample: Serum -
Food & Function (2022) IF: 6.317
Arthrospira platensis (Spirulina) fortified functional foods ameliorates iron and protein malnutrition by improving growth, modulating oxidative stress and gut microbiota in ratsDOI: 10.1039/D2FO02226E
Sample: Serum