Background

Oxidative stress has been implicated in a number of diseases such as atherosclerosis¹, chronic inflammatory disease², chronic renal failure³, and cancer⁴. such as enzymes and variously functionalized molecules (see examples below) that effectively neutralize these damaging species. These antioxidants can be either water or lipid soluble, and are localized transiently throughout various tissues, cells and cell types.
-11.
>Assay Principles

The reduction potential of the sample or standard effectively converts Cu⁺² to Cu⁺¹, thus changing the ion:1 complex with the chromogenic reagent with an absorption maximum at ca. 450 nm^12-16. A known concentration of uric acid is used to create a calibration curve, with the data being expressed as mM uric acid equivalents or in μM copper reducing equivalents.

Typical Standard Curve

>References

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