Reagents and instruments for immunology, cell biology and molecular biology.

Thiamine (hydrochloride) [67-03-8]

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Cat# HY-N0680-1g

Size : 1g

Brand : MedChemExpress

Thiamine hydrochloride (Thiamine chloride hydrochloride) is an essential micronutrient needed as a cofactor for many central metabolic enzymes.

For research use only. We do not sell to patients.

Thiamine hydrochloride Chemical Structure

CAS No. : 67-03-8

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of Thiamine hydrochloride:

Thiamine monochloride In-stock
Thiamine nitrate In-stock
Thiamine monochloride-¹³C₄ hydrochloride Get quote
Thiamine-d₃ hydrochloride Get quote
Thiamine-d₄ hydrochloride Get quote
Thiamine-¹³C₃ hydrochloride Get quote
Thiamine hydrochloride (Standard) Get quote

Description

Thiamine hydrochloride (Thiamine chloride hydrochloride) is an essential micronutrient needed as a cofactor for many central metabolic enzymes.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Molecular Weight

337.27

Formula

C₁₂H₁₈Cl₂N₄OS

CAS No.

67-03-8

Appearance

Solid

Color

White to off-white

SMILES

CC1=C(CCO)SC=[N+]1CC2=CN=C(C)N=C2N.[Cl-].Cl

Structure Classification

Others

Initial Source

Endogenous metabolite
Human Gut Microbiota Metabolites

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (296.50 mM; Need ultrasonic)

DMSO : 6.67 mg/mL (19.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9650 mL	14.8249 mL	29.6498 mL
5 mM	0.5930 mL	2.9650 mL	5.9300 mL
10 mM	0.2965 mL	1.4825 mL	2.9650 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.71 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.67 mg/mL (1.99 mM); Clear solution

This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.67 mg/mL (1.99 mM); Clear solution

This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 33.33 mg/mL (98.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (271 KB) SDS (394 KB)

COA (249 KB) HNMR (255 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Moulin M, et al. Analysis of Chlamydomonas thiamin metabolism in vivo reveals riboswitch plasticity. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):14622-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.9650 mL	14.8249 mL	29.6498 mL	74.1246 mL
	5 mM	0.5930 mL	2.9650 mL	5.9300 mL	14.8249 mL
	10 mM	0.2965 mL	1.4825 mL	2.9650 mL	7.4125 mL
	15 mM	0.1977 mL	0.9883 mL	1.9767 mL	4.9416 mL
H₂O	20 mM	0.1482 mL	0.7412 mL	1.4825 mL	3.7062 mL
	25 mM	0.1186 mL	0.5930 mL	1.1860 mL	2.9650 mL
	30 mM	0.0988 mL	0.4942 mL	0.9883 mL	2.4708 mL
	40 mM	0.0741 mL	0.3706 mL	0.7412 mL	1.8531 mL
	50 mM	0.0593 mL	0.2965 mL	0.5930 mL	1.4825 mL
	60 mM	0.0494 mL	0.2471 mL	0.4942 mL	1.2354 mL
	80 mM	0.0371 mL	0.1853 mL	0.3706 mL	0.9266 mL
	100 mM	0.0296 mL	0.1482 mL	0.2965 mL	0.7412 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Thiamine hydrochloride Related Classifications

Neurological Disease

Metabolic Enzyme/Protease Apoptosis Anti-infection
Endogenous Metabolite Apoptosis HBV Bacterial

Thiamine (hydrochloride) [67-03-8]

Cat# HY-N0680-1g

Brand : MedChemExpress

Other Forms of Thiamine hydrochloride:

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Thiamine hydrochloride Related Classifications

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