Reduced Glutathione (GSH) Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | GSH |
Sample type | Serum,plasma,cell culture supernatant,tissue,cells |
Sensitivity | 2 μmol/L |
Detection range | 2-100 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 25 min |
Precision | Average inter-assay CV: 3.200%Average intra-assay CV: 1.900% |
Other instruments required | Micropipettor, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
Images
Significant differences can be observed comparing with P group. (*P<0.05, **P
G G Liou et al found that N-acetyl cysteine can reduce propacetamol-induced hepatotoxicity with low dose, but induce with high dose. Reduced glutathione level of mouse liver tissue was determined using GSH colorimetric assay kit (E-BC-K030-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Human plasma | 1 |
10% Mouse brain tissue homogenization | 1 |
10% Mouse liver tissue homogenization | 1 |
Hela cell homogenization(0.999 mgprot/mL) | 1 |
Rat serum | 1 |
Rat plasma | 1 |
Mouse serum | 1 |
10% Carrot tissue homogenization | 1 |
293T supernatant | 1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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Pharmaceuticals (2021) IF: 5.863
Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male MiceDOI: 10.3390/ph15010016
Sample: Serum -
Biomedicines (2021) IF: 6.081
WIN55,212-2 Attenuates Cognitive Impairments in AlCl3 + d-Galactose-Induced Alzheimer’s Disease Rats by Enhancing Neurogenesis and Reversing Oxidative StressSample: Brain -
MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 6.575
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory PyroptosisPMID: 36069237
Sample: Colon Tissue -
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
Antidiabetic effects of Psidium x durbanensis Baijnath & Ramcharun ined. (Myrtaceae) leaf extract on streptozotocin-induced diabetes in ratsPMID: 35842177
Sample: Liver -
FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathwayPMID: 35489465
Sample: Pig Testicular Cell (St Cell) -
Journal of Evidence-Based Integrative Medicine (2019)
Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats:Sample: Pancreatic,Liver -
Toxicology Reports (2022)
Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar ratsSample: Plasma -
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY (2019) IF: 0.967
Bombyx mori used as a fast detection model of liver melanization after a clinical drug – Acetaminophen exposureSample: Plasma,Tissue Homogenate -
ANNALS OF VASCULAR SURGERY (2020) IF: 1.125
Which Distant Organ is Most Affected by Lower Extremity Ischemia-Reperfusion?PMID: 31927058
Sample: Tissue Homogenate -
JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
Biochemical and histopathological assessment of liver in a rat model of metabolic syndrome induced by high‐carbohydrate high‐fat dietDOI: 10.1111/jfbc.13371
Sample: Tissue Homogenate -
Molecular Medicine Reports (2018) IF: 1.922
Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosisSample: Serum -
ENVIRONMENTAL MONITORING AND ASSESSMENT (2019) IF: 1.959
Biochemical investigation of association of arsenic exposure with risk factors of diabetes mellitus in Pakistani population and its validation in animal modelPMID: 31346790
Sample: Blood,Urine -
Biomolecules & Therapeutics (2017) IF: 2.075
Hepaprotective Effect of Standardized Ecklonia stolonifera Formulation on CCl4-Induced Liver Injury in Sprague-Dawley RatsPMID: 29129047
Sample: Tissue Homogenate -
JOURNAL OF MOLECULAR HISTOLOGY (2020) IF: 2.531
Oxidative stress induced pyroptosis leads to osteogenic dysfunction of MG63 cellsPMID: 32356234
Sample: Cell Culture Supernatant -
Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling PathwayDOI: 10.1155/2021/2073296
Sample: Beas-2B Cell -
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 DiabetesDOI: 10.1155/2022/2814196
PMID: 35502173
Sample: Plasma -
JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in ratsDOI: 10.1111/jfbc.13865
PMID: 34263474
Sample: Liver,Kidney -
Applied Sciences-Basel (2023) IF: 2.838
Protective Effect of Ethanolic Extract of Djulis Hull on Indomethacin-Induced Gastric InjuryDOI: 10.3390/app13010594
Sample: Gastric Tissue -
Analytical Methods (2022) IF: 2.896
Evaluate the Effect of nitrate and chloride on Cd(II)-Induced Single Cell Oxidative Stress by Scanning Electrochemical MicroscopyDOI: 10.1039/D2AY00495J
Sample: Mcf-7 Cell -
Oncology Letters (2023) IF: 3.111
HIF‑1α inhibits ferroptosis and promotes malignant progression in non‑small cell lung cancer by activating the Hippo‑YAP signalling pathwaySample: A549 Cell -
EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damageDOI: 10.1111/ejn.14662
Sample: Tissue Homogenate -
NEUROSCIENCE LETTERS (2022) IF: 3.197
Dexmedetomidine prevents hemorrhagic brain injury by reducing damage induced by ferroptosis in micePMID: 35995304
Sample: Brain -
Animals (2023) IF: 3.231
The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin StressDOI: 10.3390/ani13030363
Sample: Plasma -
Animals (2022) IF: 3.231
Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female DromedariesDOI: 10.3390/ani12162125
PMID: 36009715
Sample: Serum -
Biomed Research International (2022) IF: 3.246
Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative StressDOI: 10.1155/2022/9943598
PMID: 35818443
Sample: Hippocampal Tissue -
Biomed Research International (2021) IF: 3.411
Antioxidant Activity Derived from Marine Green-Lipped Mussel Perna canaliculus Extracts in MiceDOI: 10.1155/2021/1622270
PMID: 34409099
Sample: Liver,Kidney,Cardiac -
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathwayPMID: 35635784
Sample: Brain Tissue -
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639
Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive ratsDOI: 10.1002/jsfa.11611
PMID: 34708426
Sample: Renal Cortex -
LIFE SCIENCES (2020) IF: 3.647
Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesisPMID: 32220623
Sample: Tissue Homogenate -
NEUROSCIENCE (2023) IF: 3.708
HC067047 Ameliorates Sepsis-associated Encephalopathy by Suppressing Endoplasmic Reticulum Stress and Oxidative Stress-Induced Pyroptosis in the Hippocampi of MiceSample: Hippocampi -
PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72
Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in ArabidopsisPMID: 32097848
Sample: Plant Tissue -
CELLULAR SIGNALLING (2020) IF: 3.968
Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axisPMID: 33370579
Sample: Sk-N-Sh Cell,Imr-32 Cell -
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental ratsSample: Testicular Tissue -
JOURNAL OF INORGANIC BIOCHEMISTRY (2021) IF: 4.155
Anti-metastasis and anti-proliferation effect of mitochondria-accumulating ruthenium(II) complexes via redox homeostasis disturbance and energy depletionPMID: 33578250
Sample: Cell Lysate -
Foods (2022) IF: 4.35
Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal MicrobiotaSample: Colon Tissue -
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathwayPMID: 34555451
Sample: Lung Tissue -
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in ratsPMID: 35443217
Sample: Serum -
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cellsPMID: 35181488
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International Journal of Nanomedicine (2018) IF: 4.37
Tanshinol borneol ester on nanostructured lipid carriers has longer brain and systemic effector retention and better antioxidant activity in vivoDOI: 10.2147/IJN.S159789
PMID: 29695905
Sample: Tissue Homogenate -
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019) IF: 4.527
Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain enzyme activity and immunoexpression of astrocyte in male ratsPMID: 31279280
Sample: Tissue Homogenate -
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino ratsPMID: 31972360
Sample: Tissue Homogenate -
MOLECULES (2022) IF: 4.927
Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in RatsPMID: 36235007
Sample: Plasma -
Antioxidants (2020) IF: 5.014
Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly SubjectsPMID: 32012711
Sample: Plasma -
Reproductive Biology and Endocrinology (2022) IF: 5.211
Neuroscience Research (2019)PMID: 35597951
Sample: Testis Tissue -
Food Bioscience (2022) IF: 5.318
Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in miceSample: Livers -
Food & Function (2021) IF: 5.396
Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive ratsDOI: 10.1039/D1FO03577K
PMID: 34994761
Sample: Renal -
Foods (2022) IF: 5.561
Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal MicrobiotaPMID: 36230124
Sample: Serum -
Foods (2022) IF: 5.561
Freshwater Clam Extract Attenuates Indomethacin-Induced Gastric Damage In Vitro and In VivoSample: Gastric Mucosa -
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
Notoginsenoside R1 induces oxidative stress and modulates LPS induced immune microenvironment of nasopharyngeal carcinomaPMID: 36279666
Sample: Npc Cells -
INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
HC067047 as a potent TRPV4 inhibitor repairs endotoxemia colonic injurySample: Colon -
Frontiers in Oncology (2022) IF: 5.738
ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.Sample: Achn Cell -
Frontiers in Pharmacology (2021) IF: 5.811
Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade. -
Frontiers in Pharmacology (2022) IF: 5.988
Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.Sample: Testicular Tissue -
Cell Death & Disease (2020) IF: 6.304
Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overloadSample: Heart -
Antioxidants (2022) IF: 6.313
Potential Neurotoxic Effects of Glioblastoma-Derived Exosomes in Primary Cultures of Cerebellar Neurons via Oxidant Stress and Glutathione DepletionPMID: 35883716
Sample: T98G Cell,U373 Cell -
Antioxidants (2021) IF: 6.313
N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal MicePMID: 33809388
Sample: Tissue Homogenate -
INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
Carboxymethyl cellulose/sericin-based hydrogels with intrinsic antibacterial, antioxidant, and anti-inflammatory properties promote re-epithelization of diabetic wounds in ratsPMID: 36280221
Sample: Skin Tissues -
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formationPMID: 34891114
Sample: Serum -
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed ratsPMID: 34399203
Sample: Heart -
Cells (2021) IF: 6.6
Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic RatsPMID: 34685668
Sample: Plasma -
Frontiers in Plant Science (2022) IF: 6.627
Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanismPMID: 35909740
Sample: Roots Or Shoots -
Nutrients (2022) IF: 6.706
UPLC-MS/MS Analysis of Naturally Derived Apis mellifera Products and Their Promising Effects against Cadmium-Induced Adverse Effects in Female RatsDOI: 10.3390/nu15010119
Sample: Ovarian Tissues -
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling PathwayDOI: 10.1155/2022/3593294
PMID: 36238648
Sample: Hippocampus -
Antioxidants (2022) IF: 7.675
Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox HomeostasisPMID: 36358477
Sample: Liver,Serum -
Antioxidants (2022) IF: 7.675
Resistance Training Modulates Reticulum Endoplasmic Stress, Independent of Oxidative and Inflammatory Responses, in Elderly PeopleSample: Pbmcs -
Antioxidants (2022) IF: 7.675
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome RatsPMID: 36290602
Sample: Ovarian Tissue -
SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoidsPMID: 34571217
Sample: Liver Organoids (Los) -
Acta Biomaterialia (2022) IF: 8.947
An all-in-one biomimetic iron-small interfering RNA nanoplatform induces ferroptosis for cancer therapyPMID: 35709941
Sample: Cal-27 Cell -
SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
Size-dependent acute toxicity and oxidative damage caused by cobalt-based framework (ZIF-67) to Photobacterium phosphoreumPMID: 36037900
Sample: T3 Strain -
CHEMICAL ENGINEERING JOURNAL (2022) IF: 16.744
Tumor microenvironment activated nanoreactors for chemiluminescence imaging-guided simultaneous elimination of breast tumors and tumor-resident intracellular pathogensSample: Mcf-7 Cells