Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot analysis of RT-4 cell lysate with RBM3 monoclonal antibody, clone CL0296 (Cat # MAB15594).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with RBM3 monoclonal antibody, clone CL0296 (Cat # MAB15594) shows moderate nuclear positivity in tumor cells.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent staining of U-2 OS cells with RBM3 monoclonal antibody, clone CL0296 (Cat # MAB15594) (Green) shows specific staining in the nucleoplasm. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against partial recombinant human RBM3.

    Immunogen

    Recombinant protein corresponding to human RBM3.

    Sequence

    DEQALEDHFSSFGPISEVVVVKDRETQRSRGFGFITFTNPEHASVAMRAMNGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDSRPGGYGYGYGRSRDYNGRNQGGYDRYSGGNY

    Host

    Mouse

    Reactivity

    Human

    Form

    Liquid

    Purification

    Protein A purification

    Isotype

    IgG1

    Recommend Usage

    Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western Blot analysis of RT-4 cell lysate with RBM3 monoclonal antibody, clone CL0296 (Cat # MAB15594).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with RBM3 monoclonal antibody, clone CL0296 (Cat # MAB15594) shows moderate nuclear positivity in tumor cells.

    Immunofluorescence

    Immunofluorescent staining of U-2 OS cells with RBM3 monoclonal antibody, clone CL0296 (Cat # MAB15594) (Green) shows specific staining in the nucleoplasm. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
  • Gene Info — RBM3

    Entrez GeneID

    5935

    Protein Accession#

    P98179

    Gene Name

    RBM3

    Gene Alias

    IS1-RNPL, RNPL

    Gene Description

    RNA binding motif (RNP1, RRM) protein 3

    Omim ID

    300027

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq

    Other Designations

    OTTHUMP00000025800|OTTHUMP00000025802|RNA binding motif protein 3

  • Interactomes
  • Publication Reference