Protein G SepFast
Cat# 230501-250ML
Size : 250ml
Brand : BioToolomics
Introduction
Protein G SepFast is a family of affinity resins with protein G immobilized to highly cross-linked agarose-based beads of various sizes.
- Protein G SepFast HighRes: 20 – 50 µm, good for small column run at shorter residence time (i.e. higher flowrate)
- Protein G SepFast: 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
- Protein G SepFast Large Bead: 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure)
Protein G binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of immunoglobins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.
The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.
Protein G SepFast is developed and supported for production scale chromatography. BioToolomics offers both loose medium and pre-packed ready-to-use disposable columns.
Characteristics of Protein G SepFast Range
Matrix | Highly cross-linked agarose bead |
Particle size | 20 - 50 µm Protein G SepFast HighRes50 - 150 µm Protein G SepFast 150 - 350 µm Protein G SepFast Large Bead |
Max. operating pressure | 0.3 MPa (3 bar, 42 psi) |
Recommended flow rate* | 50 to 300 cm/h |
Binding capacity** | ≥ 15 mg human IgG/mL resin |
Chemical compatibility | The commonly used reagents for antibody purifications |
Chemical stability | Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11. |
Physical stability | Negligible volume variation due to changes in pH or ionic strength. |
pH stability | 3 to 9 (long term); 2 to 10 (short term) |
Sanitization | Sanitize the column with 70% ethanol |
Storage & temperature | 20% ethanol at 2oC – 8oC |
*Other column size available on request.