Reagents and instruments for immunology, cell biology and molecular biology.

Prednisone [53-03-2]

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Cat# HY-B0214-500mg

Size : 500mg

Brand : MedChemExpress

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Prednisone (Adasone) is a corticosteroid agent with anti-inflammatory and immunosuppressive effects that can be used to study diseases related to systemic lupus erythematosus.

For research use only. We do not sell to patients.

Prednisone Chemical Structure

CAS No. : 53-03-2

This product is a controlled substance and not for sale in your territory.

Based on 8 publication(s) in Google Scholar

Other Forms of Prednisone:

Prednisone acetate In-stock
Prednisone-d₈ Get quote
Prednisone-d₄ Get quote
Prednisone (Standard) Get quote

Description

Prednisone (Adasone) is a corticosteroid agent with anti-inflammatory and immunosuppressive effects that can be used to study diseases related to systemic lupus erythematosus^[1]^[2].

In Vivo

Prednisone (intramuscular injection, 10 mg/kg once a day, on days 4 to 13) reduces the survival rate on days lower than the control group in BALB/c mice with encephalomyocarditis virus myocarditis. Besides, myocardial virus titers reaches a maximum on day 4, but there is no antibody titer, and on day 8 virus titers remains elevated and antibody titers is also high. On day 10, antibody titers are significantly lower than controls, while viral titers remains significantly elevated^[2].
Prednisone (5 mg/kg, intragastrically administration, daily) can cause changes in FA metabolism in SLE mouse (MRL/lpr) model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female MRL/lpr mice^[3]
Dosage:	5 mg/kg
Administration:	intragastrically administration, daily
Result:	Elevated polyunsaturated FA, such as arachidonic acid and docosahexaenoic acid, and reduced the total level of n-6 polyunsaturated fatty acidsin.

Clinical Trial

Molecular Weight

358.43

Formula

C₂₁H₂₆O₅

CAS No.

53-03-2

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1(C2)[C@](C(CO)=O)(O)CC[C@@]1(12)[C@]3(12)CCC4=CC(C=C[C@]4(C)[C@@]3(12)C2=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (348.74 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (2.79 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7899 mL	13.9497 mL	27.8995 mL
5 mM	0.5580 mL	2.7899 mL	5.5799 mL
10 mM	0.2790 mL	1.3950 mL	2.7899 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (278 KB) SDS (419 KB)

COA (252 KB) HNMR (255 KB) RP-HPLC (241 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. RIEMER AD. Application of the newer corticosteroids to augment diuresis in congestive heart failure. Am J Cardiol. 1958 Apr;1(4):488-96. [Content Brief]

[2]. [2]N Tomioka, et al. Effects of prednisolone on acute viral myocarditis in mice. J Am Coll Cardiol. 1986 Apr;7(4):868-72. [Content Brief]

[3]. Qianqian Li, et al. Metabolic Profiling Reveals an Abnormal Pattern of Serum Fatty Acids in MRL/lpr Mice Under Treatment With Prednisone. Front Pharmacol. 2020 Feb 25;11:115. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.7899 mL	13.9497 mL	27.8995 mL	69.7486 mL
DMSO	5 mM	0.5580 mL	2.7899 mL	5.5799 mL	13.9497 mL
	10 mM	0.2790 mL	1.3950 mL	2.7899 mL	6.9749 mL
	15 mM	0.1860 mL	0.9300 mL	1.8600 mL	4.6499 mL
	20 mM	0.1395 mL	0.6975 mL	1.3950 mL	3.4874 mL
	25 mM	0.1116 mL	0.5580 mL	1.1160 mL	2.7899 mL
	30 mM	0.0930 mL	0.4650 mL	0.9300 mL	2.3250 mL
	40 mM	0.0697 mL	0.3487 mL	0.6975 mL	1.7437 mL
	50 mM	0.0558 mL	0.2790 mL	0.5580 mL	1.3950 mL
	60 mM	0.0465 mL	0.2325 mL	0.4650 mL	1.1625 mL
	80 mM	0.0349 mL	0.1744 mL	0.3487 mL	0.8719 mL
	100 mM	0.0279 mL	0.1395 mL	0.2790 mL	0.6975 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Prednisone Related Classifications

Inflammation/Immunology Endocrinology Cancer
Cancer Immunotherapy Cancer Metabolism and Metastasis

Immunology/Inflammation Vitamin D Related/Nuclear Receptor Apoptosis
Glucocorticoid Receptor Apoptosis

Prednisone [53-03-2]

Cat# HY-B0214-500mg

Brand : MedChemExpress

Other Forms of Prednisone:

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Prednisone Related Classifications

Keywords:

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