Nitric Oxide (NO) Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | NO |
Sample type | Serum,plasma,tissue |
Sensitivity | 0.97 μmol/L |
Detection range | 0.97-700 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 80 min |
Precision | Average inter-assay CV: 5.200%Average intra-assay CV: 3.400% |
Other instruments required | Vortex mixer, Centrifuge, Analytical Balance, Micropipettor |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | Reagent 6: -20℃, others: 2-8℃ |
Valid period | 12 months |
Images
NO level was significantly reduced by cold exposure. (P<0.01)
BYWng et al investigate the mechanism between cold exposure, gut microbiota and hypertension. Nitric oxide (NO) level of rat plasma was determined using NO colorimetric assay kit (E-BC-K035-S).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-700 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Human plasma | 1 |
10% Mouse liver tissue homogenization | 1 |
Rat serum | 1 |
Rat plasma | 1 |
10% Epipremnum aureum tissue homogenization | 1 |
Note: The diluent is double distilled water, normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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