WESTAR SUN is our entry level ECL substrate with stable light output for mid picogram detection level.
The formulation provides a low background for a high signal to noise ratio.
NeoPRO Nano Western ECL Substrate
Cat# NB-78-00001
Size : 2x125mLkit
Brand : Neo Biotech
Properties for WESTAR SUN
| Description | WESTAR SUN is our entry level ECL substrate with stable light output for mid picogram detection level. The formulation provides a low background for a high signal to noise ratio. |
| Features | Mid picogram detection Long signal duration Working solution stable for at least five days An entry level ECL substrate with excellent performance/ ratio |
| Components | Luminol/enhancer solution (A) Peroxide solution (B) |
| Antibody Dilution Range | Primary: 1:100 - 1:5.000 Secondary: 1:1.000 - 1:15.000 (from 1 mg/mL stock solution) |
| Available as | 2 x 125 mL kit (2500 cm2 of membrane) |
| Storage | Stable for 1 year at RT Product is shipped at ambient temperature |
Online Resources & Supporting Documents
Gallery
Western Blotting Detection of ERK1/2 on HeLa cells lysates
Hela cell lysate from 10 µg to 0,15 µg
Ab 1° Rabbit anti ERK-1/2 1:1000
Ab 2° Donkey anti rabbit 1:5000
Exposure time: 90 seconds
Imager: Westar R (Hi Tech Cyanagen)
Hela cell lysate from 10 µg to 0,15 µg
Ab 1° Rabbit anti ERK-1/2 1:1000
Ab 2° Donkey anti rabbit 1:5000
Exposure time: 90 seconds
Imager: Westar R (Hi Tech Cyanagen)