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> Myc-Tag Monoclonal Antibody

Myc-Tag Monoclonal Antibody

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Cat# E-AB-48023-25

Size : 25uL

Brand : Elabscience

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Verified Samples Verified Samples in WB: HEK 293
Verified Samples in FCM: CHO
Dilution WB 1:2000-4000,  FCM 0.2μg
Isotype IgG2a
Host Mouse
Reactivity All
Applications WB,  FCM
Clonality Monoclonal
Immunogen Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
Abbre Myc-Tag
Synonyms Myc Epitope Tag,  Myc proto-oncogene protein,  Transcription factor p64,  c Myc,  c Myc Epitope Tag,  c Myc Tag
Calculated MW 28 kDa
Observed MW 28 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Concentration 1 mg/mL
Buffer PBS, pH 7.2. Contains 0.05% proclin 300.
Purification Method Protein A/G Purification
Clone No. 3E9
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background The MYC tag, derived from the c-MYC protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
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E-AB-1011 Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1015 Goat Anti-Mouse IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1059 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1076 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1087 Elab Fluor® Violet 450-Goat Anti-Mouse IgG(H+L) 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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