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> Mix-n-Stain™ CF®488A Antibody Labeling Kit, 1x(5-20ug) labeling

Mix-n-Stain™ CF®488A Antibody Labeling Kit, 1x(5-20ug) labeling

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Cat# 92273

Size : 1kit

Brand : Biotium

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Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Label your IgG antibody with one of our superior CF® Dyes in as little as 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

Overview of Mix-n-Stain Labeling Protocol

Technology

CF® Dyes
CF® Dyes for Multi-Color Super-Resolution Microscopy
Antibody & Protein Labeling Kits
Protocols
SDS
Supporting Documents

Product Description

Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your IgG antibody in as little as 15 minutes, with minimal hands-on time and no purification.

Note: This kit is for labeling IgG antibodies only. For labeling IgM antibodies, please see our Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Features

  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of over 30 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 15 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ Storage Buffer
Overview of Mix-n-Stain Labeling Protocol
Overview of the Mix-n-Stain™ Antibody Labeling Kit workflow. Click to enlarge.

Unrivaled Convenience

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Superior CF® Dyes

These kits feature a choice of 31 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choose the Right Labeling Kit for Your Antibody

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.

Other Labeling Kits

We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of IgG antibody with CF® Dyes, as well as Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Conjugation Ex/Em Size Catalog No. Dye Features
CF®350 347/448 nm 5-20 ug 92270 CF®350 Features
20-50 ug 92250
50-100 ug 92230
CF®405S 404/431 nm 5-20 ug 92271 CF®405S Features
20-50 ug 92251
50-100 ug 92231
CF®405M 408/452 nm 5-20 ug 92272 CF®405M Features
20-50 ug 92252
50-100 ug 92232
CF®405L 395/545 nm 5-20 ug 92303 CF®405L Features
20-50 ug 92304
50-100 ug 92305
3x(5-20 ug) 92445
CF®430 426/498 nm 5-20 ug 92316 CF®430 Features
20-50 ug 92317
50-100 ug 92318
CF®440 440/515 nm 5-20 ug 92319 CF®440 Features
20-50 ug 92320
50-100 ug 92321
CF®450 450/538 nm 5-20 ug 92322 CF®450 Features
20-50 ug 92323
50-100 ug 92324
CF®488A 490/515 nm 5-20 ug 92273 CF®488A Features
20-50 ug 92253
50-100 ug 92233
3x(5-20 ug) 92446
CF®514 516/548 nm 5-20 ug 92331 CF®514 Features
20-50 ug 92332
50-100 ug 92333
CF®532 527/558 nm 5-20 ug 92289 CF®532 Features
20-50 ug 92290
50-100 ug 92291
CF®543 541/560 nm 5-20 ug 92287 CF®543 Features
20-50 ug 92267
50-100 ug 92247
CF®555 555/565 nm 5-20 ug 92274 CF®555 Features
20-50 ug 92254
50-100 ug 92234
CF®568 562/583 nm 5-20 ug 92275 CF®568 Features
20-50 ug 92255
50-100 ug 92235
3x(5-20 ug) 92447
CF®570 568/591 nm 5-20 ug 92334 CF®570 Features
20-50 ug 92335
50-100 ug 92336
CF®583 583/606 nm 5-20 ug 92337 CF®583 Features
20-50 ug 92338
50-100 ug 92339
CF®583R 586/609 nm 5-20 ug 92455 CF®583R Features
20-50 ug 92456
50-100 ug 92457
CF®594 593/614 nm 5-20 ug 92276 CF®594 Features
20-50 ug 92256
50-100 ug 92236
3x(5-20 ug) 92448
CF®597R 597/619 nm 5-20 ug 92461 CF®597R Features
20-50 ug 92462
50-100 ug 92463
CF®633 630/650 nm 5-20 ug 92277 CF®633 Features
20-50 ug 92257
50-100 ug 92237
CF®640R 642/662 nm 5-20 ug 92278 CF®640R Features
20-50 ug 92258
50-100 ug 92245
CF®647 650/665 nm 5-20 ug 92279 CF®647 Features
20-50 ug 92259
50-100 ug 92238
3x(5-20 ug) 92449
CF®660R 663/682 nm 5-20 ug 92281 CF®660R Features
20-50 ug 92261
50-100 ug 92243
CF®660C 667/685 nm 5-20 ug 92280 CF®660C Features
20-50 ug 92260
50-100 ug 92239
CF®680 681/698 nm 5-20 ug 92282 CF®680 Features
20-50 ug 92262
50-100 ug 92240
CF®680R 680/701 nm 5-20 ug 92283 CF®680R Features
20-50 ug 92263
50-100 ug 92246
CF®700 695/720 nm 5-20 ug 92425 CF®700 Features
20-50 ug 92426
50-100 ug 92427
CF®750 755/777 nm 5-20 ug 92284 CF®750 Features
20-50 ug 92264
50-100 ug 92241
CF®770 770/797 nm 5-20 ug 92285 CF®770 Features
20-50 ug 92265
50-100 ug 92242
CF®790 784/806 nm 5-20 ug 92288 CF®790 Features
20-50 ug 92268
50-100 ug 92248
CF®800 797/816 nm 5-20 ug 92428 CF®800 Features
20-50 ug 92429
50-100 ug 92430
CF®820 822/835 nm 5-20 ug 92431 CF®820 Features
20-50 ug 92432
50-100 ug 92433
* Download the Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

Antibody & Protein Labeling Kits

Product
Name		 Label
Options		 Labeling
Scale		 Labeling
Time		 Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits		 CF® Dyes ≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG		 ~ 15 min. • Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits		 FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits		 Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits		 Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits		 Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits		 DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits		 CF® Dyes
Cyanine Dyes		 1 mg IgG ~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits CF® Dyes
FITC		 25 ug IgM
100 ug IgM		 15-30 min. • Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits CF® Dyes 50 ug IgG ~ 30 min. • Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits		 CF® Dyes 5-20 ug Nanobody®
20-50 ug Nanobody®		 ~ 30 min. • Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits		 CF® Dyes 0.1 umol
small ligand		 ~ 30 min. • Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging Near-IR CF® Dyes 3 x 1 mg IgG ~ 2 hours • Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits		 CF® Dyes
Biotin		 3 x 1 mg
protein		 ~ 2 hours • Dyes, buffers, & spin vials for labeling + purification
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of CF® Dye and Mix-n-Stain™ references.

 

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