KLN-205 genomic DNA - 5 microgram
Cat# 400419GD5
Size : 5ug
Brand : CLS Cell Lines Service
KLN-205 Cells
General information
Description | KLN-205 is a murine lung carcinoma cell line derived from an adult mouse. This cell line is widely used in cancer research, particularly for studying the mechanisms of lung cancer progression, metastasis, and potential therapeutic interventions. KLN-205 cells exhibit characteristics typical of non-small cell lung carcinoma (NSCLC), making them a valuable model for investigating the molecular and cellular underpinnings of this disease. Researchers utilize KLN-205 to evaluate the efficacy of various chemotherapeutic agents, immunotherapies, and targeted treatments, helping to advance the understanding of lung cancer biology and treatment strategies. KLN-205 cells are known for their robust growth and ability to form tumors when implanted in immunocompromised mice, providing a reliable in vivo model for preclinical studies. These cells are used to explore tumor-host interactions, immune responses to lung cancer, and the impact of genetic and epigenetic modifications on cancer development and progression. The KLN-205 cell line serves as a critical tool in oncology research, aiding in the identification of novel biomarkers and therapeutic targets for lung cancer. |
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Organism | Mouse |
Tissue | Lung |
Disease | Squamous cell carcinoma |
Synonyms | KLN 205, KLN205 |
Characteristics
Growth properties | Adherent |
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Identifiers / Biosafety / Citation
Citation | KLN-205 (Cytion catalog number 400419) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in DBA/2 and BDF1 mice |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 degree Celsius for 10 -15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Split ratio | A ratio of 1:5 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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