Iron Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | Fe |
Sample type | Serum,tissue |
Sensitivity | 0.08 mg/L |
Detection range | 0.29-10 mg/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 25 min |
Precision | Average inter-assay CV: 2.800%Average intra-assay CV: 1.200% |
Other instruments required | Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
Images
The iron levels in A549 cells were significantly increased after treatment with BPQDs. (*P<0.05, **P<0.01)
F Ruan et al investigate the nanotoxicological assessment of Black Phosphorus Quantum Dots (BPQD). Iron (Fe) level in lung A549 cells were determined using iron colorimetric assay kit (E-BC-K139-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-10 mg/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Mouse serum | 1 |
10% Mouse liver tissue homogenate | 1 |
10% Rat kidney tissue homogenate | 1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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Bioengineered (2022) IF: 3.269
Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cellsSample: Kle Cell -
TOXICOLOGY (2020) IF: 4.099
Inhibition of ferroptosis attenuates busulfan-induced oligospermia in miceSample: Testicular Tissue -
BIOLOGY OF REPRODUCTION (2021) IF: 4.285
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J miceSample: Serum,Ovary -
MOLECULAR IMMUNOLOGY (2021) IF: 4.407
Hydrogen sulfide attenuates ferroptosis and stimulates autophagy by blocking mTOR signaling in sepsis-induced acute lung injuryPMID: 34952420
Sample: Lung Tissue -
JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosisSample: Serum,Liver Tissue,Aml-12 Cell -
Frontiers in Oncology (2022) IF: 6.244
Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.Sample: U251 Cell,U87 Cell -
Antioxidants (2022) IF: 6.313
Hyperoxia Induces Ferroptosis and Impairs Lung Development in Neonatal MicePMID: 35453326
Sample: Lung Tissues -
Food & Function (2022) IF: 6.317
Arthrospira platensis (Spirulina) fortified functional foods ameliorates iron and protein malnutrition by improving growth, modulating oxidative stress and gut microbiota in ratsDOI: 10.1039/D2FO02226E
Sample: Serum -
International Journal of Nanomedicine (2022) IF: 6.4
A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone RepairDOI: 10.2147/IJN.S351814
PMID: 35299865
Sample: Bpn-Dfo Hydrogel -
Nutrients (2022) IF: 6.706
Iron Depletion in Systemic and Muscle Compartments Defines a Specific Phenotype of Severe COPD in Female and Male Patients: Implications in Exercise ToleranceDOI: 10.3390/nu14193929
PMID: 36235581
Sample: Muscle -
Antioxidants (2022) IF: 7.675
Cathelicidin Attenuates Hyperoxia-Induced Lung Injury by Inhibiting Ferroptosis in Newborn RatsSample: Lung Tissues -
ANTIOXIDANTS & REDOX SIGNALING (2021) IF: 8.401
A novel identified circular RNA, mmu_mmu_circRNA_0000309 involves in Germacrone-mediated the improvement of diabetic nephropathy through regulating ferroptosis by targeting miR-188-3p/GPX4 signaling axisSample: Kidney,Primary Mouse Podocyte Mpc5 -
Cell Death & Disease (2022) IF: 8.469
STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathwayPMID: 35668064
Sample: Lung Tissue,Hbe Cell -
Cell Death & Disease (2022) IF: 9.685
UHRF1-mediated ferroptosis promotes pulmonary fibrosis via epigenetic repression of GPX4 and FSP1 genesSample: Lung Tissue -
JOURNAL OF CONTROLLED RELEASE (2022) IF: 9.776
A targeted nanomodulator capable of manipulating tumor microenvironment against metastasisPMID: 35716882
Sample: 4 T1 Cell-Xenograft -
Small Methods (2021) IF: 14.188
RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological AssessmentPMID: 34927824
Sample: Cell Sample