Human PYY (Peptide YY) CLIA Kit

Cat# E-CL-H0803-48T

Size : 48T

Brand : Elabscience

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Product Summary
Sensitivity 4.69 pg/mL
Detection Range 7.81-500 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes Human PYY in samples.No significant cross-reactivity or interference between Human PYY and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Chemiluminescence
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 6 months
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PYY. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PYY. You can calculate the concentration of Human PYY in the samples by comparing the RLU value of the samples to the standard curve.
Uniport ID P10082
Research Area Cancer , Metabolism , Neuroscience
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