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Application Data with Unconjugated Antibody
Western Blot (Tissue lysate)
Western blot analysis of rat skeletal muscle tissue with HSPA1A monoclonal antibody, clone 5A5 (Cat # MAB11390).
- Specifications
Product Description
Mouse monoclonal antibody raised against recombinant HSPA1A.
Immunogen
Recombinant protein corresponding to human HSPA1A.
Host
Mouse
Reactivity
Amphibia, Chicken, Fish, Fruit fly, Human, Mouse, Rat, Yeast
Specificity
Detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, Grp78 and following heat shock, Hsp72.
Form
Liquid
Conjugation
HRP
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:500)
Immunoprecipitation (1 ug)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
Application Data with Unconjugated Antibody
Western Blot (Tissue lysate)
Western blot analysis of rat skeletal muscle tissue with HSPA1A monoclonal antibody, clone 5A5 (Cat # MAB11390).
- Applications
Western Blot (Tissue lysate)
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
- Gene Info — HSPA1A
Entrez GeneID
3303Protein Accession#
NP_005336.3Gene Name
HSPA1A
Gene Alias
FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSPA1B
Gene Description
heat shock 70kDa protein 1A
Omim ID
140550Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029287|dnaK-type molecular chaperone HSP70-1|heat shock 70kD protein 1A|heat shock 70kDa protein 1B|heat shock-induced protein
- Interactomes
- Pathways
- Diseases