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Flow Cytometry
Surface staining of human peripheral blood cells with HLA-A monoclonal antibody, clone W6/32 (Cat # MAB6449) Alexa Fluor® 488.
- Specifications
Product Description
Mouse monoclonal antibody raised against native HLA-A.
Immunogen
Native purified HLA-A from membrane of human tonsil cells.
Host
Mouse
Reactivity
Bovine, Cat, Human, Non-Human Primates
Specificity
This antibody recognizes MHC Class I molecules (MHC Class Ia) that are expressedon the surface of all human nucleated cell types. This antibody is also suitable as a positive control for HLA tissue typing andcrossmatching.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at 4°C. Do not freeze.
- Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Functional Study
Flow Cytometry
Surface staining of human peripheral blood cells with HLA-A monoclonal antibody, clone W6/32 (Cat # MAB6449) Alexa Fluor® 488. - Gene Info — HLA-A
Entrez GeneID
3105Gene Name
HLA-A
Gene Alias
HLAA
Gene Description
major histocompatibility complex, class I, A
Gene Ontology
HyperlinkGene Summary
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, A-23 alpha chain|MHC class I antigen HLA-A heavy chain|MHC leukocyte antigen|OTTHUMP00000161059|antigen presenting molecule|leucocyte antigen class I|leukocyte antigen class I-A
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Application of lyophilised human platelets for antibody detection in solid phase red cell adherence assay.
Shengbao Duan, Mingyuan Wang, Shaohua Ding, Yezhou Chen, Shuangshi Wei, Wei Chen, Chun Zhang, Yong Li, Hongmei Wang.
Journal of Immunological Methods 2020 Sep; 112868.
Application：Flow Cyt, Human, Human platelets.
- A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail.
Onishi H, Koya N, Kiyota A, Tanaka H, Umebayashi M, Katano M, Morisaki T.
Anticancer Research 2012 Jun; 32(6):2385.
Application：Func, Human, Hep-G2 cells.
- Expression of HLA-G in human cornea, an immune-privileged tissue.
Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED.
Human Immunology 2003 Nov; 64(11):1039.
Application：IHC-Fr, Human, Human Cornea.
- The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex.
Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V.
Immunogenetics 2001 Aug; 53(6):440.
Application：WB-Ce, Human, Human peripheral blood lymphocytes.
- Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody.
J J Ladasky, B P Shum, F Canavez, H N Seuanez, P Parham.
Immunogenetics 1999 Apr; 49(4):312.
Application：Flow Cyt, Monkey, OM VI-3 cells, Owl monkey 531H cells.
- Application of lyophilised human platelets for antibody detection in solid phase red cell adherence assay.