Reagents and instruments for immunology, cell biology and molecular biology.

FOX1 (Fox-1 Homolog A, Ataxin-2-binding Protein 1, A2BP1, A2BP, Hexaribonucleotide-binding Protein 1, HRNBP1, RNA Binding Protein Fox-1 Homolog 1, RBFOX1)

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Cat# 031361-100ul

Size : 100ul

Brand : US Biological

031361 Rabbit Anti-FOX1 (Fox-1 Homolog A, Ataxin-2-binding Protein 1, A2BP1, A2BP, Hexaribonucleotide-binding Protein 1, HRNBP1, RNA Binding Protein Fox-1 Homolog 1, RBFOX1) discontinued

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

Q9NWB1

Isotype

IgG1,k

Grade

Affinity Purified

Applications

IF WB

Crossreactivity

Bo Hu Mo Rt

Shipping Temp

Blue Ice

Storage Temp

-20°C

Fox1 is one of a family of 3 mammalian Fox1 homologs. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X" (1). The C. elegans Fox1 protein and its 3 mammalian homologues are all about 46kD proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2). As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3). Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5). Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6). Full length Fox1 was not used as the immunogen since the three mammalian Fox1 homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated. The HGNC name for this protein is RBFOX1.||Applications:|Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 1:500-1:1000|Western Blot: 1:1000-1:2000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1,k|Clone No: 1G10|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, 10mM sodium azide.|Purity: Purified by affinity chromatography.|Immunogen: N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli. Full length Fox1 was not used as the immunogen since the three mammalian Fox1 homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs.|Specificity: Recognizes Fox1 from human, bovine, mouse and rat. Since Fox1 is highly conserved, it is likely that the antibody is effective on other species also.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli. Full length Fox1 was not used as the immunogen since the three mammalian Fox1 homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs.

Form

Supplied as a liquid in PBS, 10mM sodium azide.

Purity

Purified by affinity chromatography.

Specificity

Recognizes Fox1 from human, bovine, mouse and rat. Since Fox1 is highly conserved, it is likely that the antibody is effective on other species also.

References

1. Hodgkin J, Zellan JD, Albertson DG. Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans. Development 120:3681-3689 (1994).||2. Shibata H, Huynh DP, Pulst SM. A novel protein with RNA-binding motifs interacts with ataxin-2. Hum Mol Genet. 39:1303-13 (2000).||3. Underwood JG, Boutz PL, Dougherty JD, Stoilov P and Black DL. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol. Cell. Biol. 25:10005-10016 (2005).||4. Mullen RJ, Buck CR, Smith AM. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116:201-211 (1994).||5. Kim KK, Adelstein RS, Kawamoto S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284:31052-31061 (2009).||6. Kim KK, Kim YC, Adelstein RS, Kawamoto S. Fox-3 and PSF interact to activate neural cell-specific alternative splicing. Nucleic Acids Res. 39:3064-78 (2011).