Reagents and instruments for immunology, cell biology and molecular biology.
 
> Ferrous Iron Colorimetric Assay Kit

Ferrous Iron Colorimetric Assay Kit

Print Print
Please log in

Cat# E-BC-K773-M-48T

Size : 48T

Brand : Elabscience


Detection principle

Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.

Performance characteristics

Sample type serum, tissue
Sensitivity 0.4 μmol/L
Detection range 0.4-50 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 1.500%Average intra-assay CV: 1.300%
Storage 2-8℃
Valid period 12 months

Images

Ferrous ion level in serum was significantly induced by CCl4 and conversed by SFN. (**P<0.01, ***P<0.001)

J W Liu et al investigate Nrf2 and its dependent autophagy activation cooperatively counteract ferroptosis to alleviate acute liver injury. Ferrous ion level in rat serum were determined using ferrous ion colorimetric assay kit (E-BC-K773-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.4-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1-2

Rat serum

1

10% Mouse liver tissue homogenate

1-3

10% Rat lung tissue homogenate

1

10% Mouse heart tissue homogenate

1

10% Rat spleen tissue homogenate

1-3

10% Epipremnum aureum leaf tissue homogenate

1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

  1. Frontiers in Pharmacology (2022) IF: 5.811
    Tetrandrine Citrate Suppresses Breast Cancer via Depletion of Glutathione Peroxidase 4 and Activation of Nuclear Receptor Coactivator 4-Mediated Ferritinophagy

    DOI: 10.3389/fphar.2022.820593
    Sample: Mcf7 Cell,Mda-Mb-231 Cell
  2. Oncology Letters (2022) IF: 2.967
    Carboxymethylated pachyman induces ferroptosis in ovarian cancer by suppressing NRF1/HO‑1 signaling

    DOI: 10.3892/ol.2022.13281
    Sample: Skov3 Cell,Hey Cell
  3. NEUROSCIENCE LETTERS (2022) IF: 3.197
    Dexmedetomidine prevents hemorrhagic brain injury by reducing damage induced by ferroptosis in mice

    DOI: 10.1016/j.neulet.2022.136842

    PMID: 35995304

    		 Sample: Brain
  4. Animals (2022) IF: 3.231
    Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis

    DOI: 10.3390/ani12213026
    Sample: Testicular Tissue
  5. Bioengineered (2021) IF: 3.269
    Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis

    DOI: 10.1080/21655979.2021.2010369

    PMID: 34903141

    		 Sample: Arpe19 Cell
  6. Nutrition & Metabolism (2022) IF: 4.654
    Association between iron metabolism and non-alcoholic fatty liver disease: results from the National Health and Nutrition Examination Survey (NHANES 2017–2018) and a controlled animal study

    DOI: 10.1186/s12986-022-00715-y
    Sample: Serum
  7. EXPERIMENTAL NEUROLOGY (2022) IF: 5.62
    S100A8 regulates autophagy-dependent ferroptosis in microglia after experimental subarachnoid hemorrhage

    DOI: 10.1016/j.expneurol.2022.114171

    PMID: 35870523

    		 Sample: Brain Tissue,Bv2 Microglia
  8. Frontiers in Oncology (2022) IF: 5.738
    Circular RNA circLMO1 Suppresses Cervical Cancer Growth and Metastasis by Triggering miR-4291/ACSL4-Mediated Ferroptosis

    DOI: 10.3389/fonc.2022.858598
    Sample: Caski Cell
  9. Frontiers in Oncology (2022) IF: 6.244
    Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.

    DOI: 10.3389/fonc.2022.859621
    Sample: U251 Cell,U87 Cell
  10. Nutrients (2023) IF: 6.706
    Lactobacillus johnsonii L531 Ameliorates Salmonella enterica Serovar Typhimurium Diarrhea by Modulating Iron Homeostasis and Oxidative Stress via the IRP2 Pathway

    DOI: 10.3390/nu15051127
    Sample: Ipec-J2 Cell
  11. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

    DOI: 10.1155/2022/3593294

    PMID: 36238648

    		 Sample: Hippocampus
  12. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization

    DOI: 10.1155/2022/9735555

    PMID: 35993021

    		 Sample: Kidney Tissue,Hk-2 Cell
  13. Cells (2022) IF: 7.666
    Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITF

    DOI: 10.3390/cells11223580
  14. eLife (2023) IF: 8.713
    Neutral amino acid transporter SLC38A2 protects renal medulla from hyperosmolarity-induced ferroptosis

    DOI: 10.7554/eLife.80647
    Sample: Renal Medullary Tissue
  15. PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
    Nrf2 and its dependent autophagy activation cooperatively counteract ferroptosis to alleviate acute liver injury

    DOI: 10.1016/j.phrs.2022.106563
    Sample: Serum
  16. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
    Inhibitory effect and mechanism of gelatin stabilized ferrous sulfide nanoparticles on porcine reproductive and respiratory syndrome virus

    DOI: 10.1186/s12951-022-01281-4

    PMID: 35123507

    		 Sample: Marc-145 Cell
  17. EBioMedicine (2022) IF: 11.205
    Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis

    DOI: 10.1016/j.ebiom.2022.104258

    PMID: 36137413

    		 Sample: Oa Cartilage
  18. Small Methods (2021) IF: 14.188
    RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment

    DOI: 10.1002/smtd.202001045

    PMID: 34927824

    		 Sample: Cell Sample