Ferrous Iron Colorimetric Assay Kit
Detection principle
Performance characteristics
Sample type | Serum, plasma, animal tissue |
Sensitivity | 0.08 mg/L |
Detection range | 0.08-20 mg/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 45 min |
Precision | Average inter-assay CV: 3.200%Average intra-assay CV: 3% |
Other instruments required | Test tubes, Vortex Mixer, Centrifuge, Water bath, Test tubes, Vortex Mixer, Centrifuge, Water bath |
Storage | 2-8℃ |
Valid period | 12 months |
Images
The administration of Fe nanoparticle significantly increased the ferrous level in cells with dose dependent.
T Tong et al developed a novel ferrous sulfide nanoparticles for inhibiting porcine reproductive and respiratory syndrome virus. Ferrous ion levels in cell were determined using ferrous ion colorimetric assay kit (E-BC-K304-S).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.08-50 mg/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human plasma | 1 |
Human serum | 1 |
10% Rat liver tissue homogenate | 1 |
10% Epipremnum aureum homogenate | 1 |
Note: The diluent is double distilled water).
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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Frontiers in Pharmacology (2022) IF: 5.811
Tetrandrine Citrate Suppresses Breast Cancer via Depletion of Glutathione Peroxidase 4 and Activation of Nuclear Receptor Coactivator 4-Mediated FerritinophagySample: Mcf7 Cell,Mda-Mb-231 Cell -
Oncology Letters (2022) IF: 2.967
Carboxymethylated pachyman induces ferroptosis in ovarian cancer by suppressing NRF1/HO‑1 signalingSample: Skov3 Cell,Hey Cell -
Bioengineered (2021) IF: 3.269
Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axisPMID: 34903141
Sample: Arpe19 Cell -
Nutrition & Metabolism (2022) IF: 4.654
Association between iron metabolism and non-alcoholic fatty liver disease: results from the National Health and Nutrition Examination Survey (NHANES 2017–2018) and a controlled animal studySample: Serum -
EXPERIMENTAL NEUROLOGY (2022) IF: 5.62
S100A8 regulates autophagy-dependent ferroptosis in microglia after experimental subarachnoid hemorrhagePMID: 35870523
Sample: Brain Tissue,Bv2 Microglia -
Frontiers in Oncology (2022) IF: 5.738
Circular RNA circLMO1 Suppresses Cervical Cancer Growth and Metastasis by Triggering miR-4291/ACSL4-Mediated FerroptosisSample: Caski Cell -
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 StabilizationDOI: 10.1155/2022/9735555
PMID: 35993021
Sample: Kidney Tissue,Hk-2 Cell -
JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
Inhibitory effect and mechanism of gelatin stabilized ferrous sulfide nanoparticles on porcine reproductive and respiratory syndrome virusPMID: 35123507
Sample: Marc-145 Cell