E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (FITC)

E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.||Applications: |Suitable for use in Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay. Other applications not tested.||Recommended Dilutions:|Western Blot: 0.5-2ug/ml of antibody detected E2F-1 in a HeLa nuclear extract. |Immunoprecipitation: 4ug immunoprecipitated E2F-1 from HeLa nuclear extract.|Optimal dilutions to be determined by the researcher.||Positive Antigen Control (Included): |H1838: HeLa Nuclear Extract||Hybridoma: |SP2 mouse myeloma cells with immunized mouse splenocytes.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.