Reagents and instruments for immunology, cell biology and molecular biology.

Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

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Cat# E-BC-K188-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle	Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.
Synonyms	Cr
Sample Type	Serum,Plasma,Urine
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (510-520 nm, optimum wavelength: 515 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	3.8 μmol/L
Detection Range	20.45-400 μmol/L
Precision	inter-assay CV: 3.7% \| intra-assay CV: 1.4%
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K016-M	Uric Acid (UA) Colorimetric Assay Kit	96T , 48T
E-BC-K183-M	Urea (BUN) Colorimetric Assay Kit (Urease Method)	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human urine	40-60
Human serum	1
Rat serum	1
Porcine serum	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.