Assay Principle

The DetectX® Cortisone ELISA Kit quantitatively measures cortisone in various samples, including serum, plasma, urine, saliva, and fecal extracts. This competitive ELISA, with a run time of 2.5 hours, offers a blend of efficiency and accuracy. Read the complete kit insert for comprehensive instructions before starting the assay. A cortisone standard is included to establish a precise standard curve.

Protocol Summary

• Add standards or diluted samples to the transparent microtiter plate provided. The plate is coated with goat anti-rabbit IgG antibody.
• Introduce cortisone peroxidase conjugate and cortisone polyclonal rabbit antibody to begin the immunological reaction.
• Incubate the mixture for 2.5 hours at room temperature with shaking, noting that the reaction inversely correlates with the cortisone concentration in the sample.
• After incubation, wash away excess conjugate and add the TMB substrate, which reacts with the bound conjugate to produce a measurable signal.
• Use a plate reader to detect the signal at 450nm and calculate cortisone concentration using the standard curve.

Background

Cortisone (C21H28O5), also known as Kendall’s Compound ‘E’, was first identified from bovine suprarenal gland tissue. Two 11β-hydroxysteroid dehydrogenase (11β-HSD) enzymes regulate the balance between cortisol and cortisone. 11β-HSD1, mainly found in the liver, converts cortisone to cortisol, whereas 11β-HSD2, present in kidney tissues, is vital for cortisol receptor binding.

This glucocorticoid shuttle is crucial in initiating and regulating anti-inflammatory responses in the body. The dynamic interplay between cortisol and cortisone is pivotal in research on stress physiology, endocrine disorders, and inflammatory conditions. Furthermore, cortisone’s role in metabolic processes and interactions with various body systems is a significant area of focus in medical research.

The DetectX® Cortisone ELISA Kit, with its adaptability and precision, is an important resource for researchers studying human physiology and pathophysiology. The kit enables accurate cortisone measurement in diverse biological samples.