Cell Counting Kit-3D (CCK-3D)
Cat# K2270-1000
Size : 1000T
Brand : APExBIO Technology
Cell Counting Kit-3D (CCK-3D)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Traditional cell culture is mainly based on 2D culture. With the deepening of cell research, scientists have found that 2D cultured cells are significantly different from cells under physiological conditions in vivo. In order to better simulate the growth of cells in the body, scientists are increasingly using 3D cell culture technology.
Cell Counting Kit-3D (CCK-3D) is convenient and sensitive for detecting cell proliferation/toxicity of 3D cells. It uses a reaction substrate of the WST series, which can be reduced by the abundant dehydrogenase in living cells in the presence of electron-coupled reagents and converted to the soluble, orange formazan in culture media. The amount of formazan produced by dehydrogenase is directly and linearly related to the number of viable cells.
This reagent is optimized for viability assays of a variety of cultured 3D spheroids, such as drop plates, ultra-low adsorption plates, Matrigel-coated and agarose-coated 3D spheroids. At the same time, this reagent is ready to use, making it ideal for high-throughput screenings.
Figure 1: Verification diagram of this product. Compared with Competitor B, the detection effect of this product is more sensitive
Protocol| Components | K2270-100 T | K2270-500 T | K2270-1000 T | K2270-3000 T | K2270-10000 T |
| Cell Counting Kit-3D (CCK-3D) | 1×1 mL | 5×1 mL | 2×5 mL | 6×5 mL | 20×5 mL |
| Store at -20°C away from light, stable for 1 year. For frequent use, keep it at 4°C away from light. | |||||