Reagents and instruments for immunology, cell biology and molecular biology.

BCA Protein Colorimetric Assay Kit

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Cat# E-BC-K318-M-500

Size : 500Assays

Brand : Elabscience

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Detection Principle	Cu²⁺ can be reduced to Cu⁺ by protein in alkaline condition. Cu⁺ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.
Synonyms	TP
Sample Type	Serum,Plasma,Cell culture supernatant,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (540-590 nm, optimum wavelength: 562 nm)
Research Area	Amino Acids And Proteins
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at room temperature for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.0165 mg/mL
Detection Range	0.0165-1 mg/mL
Precision	inter-assay CV: 4.5% \| intra-assay CV: 2.2%
Assay Time	40 min

Cat.No.	Product Name	Sizes
E-BC-K165-M	Biuret Protein Colorimetric Assay Kit	500Assays , 96T
E-BC-K168-M	Bradford Protein Colorimetric Assay Kit	500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse brain tissue homogenization	8-12
10% Mouse kidney tissue homogenization	8-12
Human serum	100-200
10% Rat liver tissue homogenization	15-20
10% Mouse heart tissue homogenization	8-12
Rat serum	100-200

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.