Reagents and instruments for immunology, cell biology and molecular biology.

Anti-PRKAB2 antibody (1-272)

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Cat# NB-22-23420-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

Immunofluorescence analysis of NIH/3T3 cells using PRKAB2 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Jurkat cells, using PRKAB2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using PRKAB2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

General Info

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-PRKAB2 (1-272) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	PRKAB2
Gene ID:	5565
Uniprot ID:	AAKB2_HUMAN
Immunogen Region:	1-272
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human PRKAB2 (NP_005390.1).
Immunogen Sequence:	MGNTTSDRVSGERHGAKAAR SEGAGGHAPGKEHKIMVGST DDPSVFSLPDSKLPGDKEFV SWQQDLEDSVKPTQQARPTV IRWSEGGKEVFISGSFNNWS TKIPLIKSHNDFVAILDLPE GEHQYKFFVDGQWVHDPSEP VVTSQLGTINNLIHVKKSDF EVFDALKLDSMESSETSCRD LSSSPPGPYGQEMYAFRSEE RFKSPPILPPHLLQVILNKD TNISCDPALLPEPNHVMLN

Description

Post Translational Modifications	Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
Function	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name	5'-Amp-Activated Protein Kinase Subunit Beta-2 Ampk Subunit Beta-2
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-1632852 Reactome: R-HSA-163680 Reactome: R-HSA-200425 Reactome: R-HSA-2151209 Reactome: R-HSA-380972 Reactome: R-HSA-5628897 Reactome: R-HSA-6804756 Reactome: R-HSA-9613354 Reactome: R-HSA-9619483
Alternative Antibody Names	Anti-5'-Amp-Activated Protein Kinase Subunit Beta-2 antibody Anti-Ampk Subunit Beta-2 antibody Anti-PRKAB2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance