Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Niemann Pick C1 antibody (1179-1278) (Clone: S8MR)

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Cat# NB-22-72785-100

Size : 100uL

Brand : Neo Biotech

Immunofluorescence analysis of PC-12 cells using Niemann Pick C1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using Niemann Pick C1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Niemann Pick C1 Rabbit monoclonal antibody at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Niemann Pick C1 Rabbit monoclonal antibody at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Niemann Pick C1 Rabbit monoclonal antibody at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Western blot analysis of extracts of various cell lines, using Niemann Pick C1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of Mouse liver, using Niemann Pick C1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-Niemann Pick C1 (1179-1278) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S8MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	NPC1
Gene ID:	4864
Uniprot ID:	NPC1_HUMAN
Immunogen Region:	1179-1278
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1179-1278 of human Niemann Pick C1 (O15118).
Immunogen Sequence:	MKGSRVERAEEALAHMGSSV FSGITLTKFGGIVVLAFAKS QIFQIFYFRMYLAMVLLGAT HGLIFLPVLLSYIGPSVNKA KSCATEERYKGTERERLLNF

Description

Post Translational Modifications	N-glycosylated.
Function	Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). Inhibits cholesterol-mediated mTORC1 activation throught its interaction with SLC38A9. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus.
Protein Name	Npc Intracellular Cholesterol Transporter 1 Niemann-Pick C1 Protein
Database Links	Reactome: R-HSA-8964038
Cellular Localisation	Late Endosome Membrane Multi-Pass Membrane Protein Lysosome Membrane
Alternative Antibody Names	Anti-Npc Intracellular Cholesterol Transporter 1 antibody Anti-Niemann-Pick C1 Protein antibody Anti-NPC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance