Reagents and instruments for immunology, cell biology and molecular biology.

Anti-ACAA2 antibody (298-397) (Clone: S4MR)

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Cat# NB-22-71913-100

Size : 100uL

Brand : Neo Biotech

Immunofluorescence analysis of U2OS cells using ACAA2 Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ACAA2 Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using ACAA2 Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ACAA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

General Info

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-ACAA2 (298-397) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	ACAA2
Gene ID:	10449
Uniprot ID:	THIM_HUMAN
Immunogen Region:	298-397
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 298-397 of human ACAA2 (P42765).
Immunogen Sequence:	PAISGALKKAGLSLKDMDLV EVNEAFAPQYLAVERSLDLD ISKTNVNGGAIALGHPLGGS GSRITAHLVHELRRRGGKYA VGSACIGGGQGIAVIIQSTA

Description

Function	In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
Protein Name	3-Ketoacyl-Coa Thiolase - Mitochondrial Acetyl-Coa Acetyltransferase Acetyl-Coa Acyltransferase Acyl-Coa Hydrolase - Mitochondrial Beta-Ketothiolase Mitochondrial 3-Oxoacyl-Coa Thiolase T1
Database Links	Reactome: R-HSA-77289
Cellular Localisation	Mitochondrion
Alternative Antibody Names	Anti-3-Ketoacyl-Coa Thiolase - Mitochondrial antibody Anti-Acetyl-Coa Acetyltransferase antibody Anti-Acetyl-Coa Acyltransferase antibody Anti-Acyl-Coa Hydrolase - Mitochondrial antibody Anti-Beta-Ketothiolase antibody Anti-Mitochondrial 3-Oxoacyl-Coa Thiolase antibody Anti-T1 antibody Anti-ACAA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance