Ampicillin sodium salt is a semi-synthetic derivative of penicillin used to select for ampicillin resistance in mutated and transformed cells. Ampicillin is a ß-lactam antibiotic that inhibits bacterial cell wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane. The antimicrobial spectrum includes gram-positive and gram-negative bacteria.
Recommended for antibacterial use in cell culture media at 100 µg/ml and in ampicillin-resistance studies at 20 - 125 μg/ml.
Solubility in water: 1 part in 2 parts H₂O. A stock solution should not be autoclaved but sterilized through filtration and stored frozen where it will be stable for months. Stability of ampicillin in solution is a function of pH, temperature and the identity of the buffer. Optimal storage conditions are 2 - 8 °C and pH 3.8 - 5 where its activity is retained at >90 % for a week.

Assay

91.0 - 102.0 %

References:

1. Davies, J. & Smith, D.J. (1978) Ann. Rev. Microbiol. 32, 469

DANGER

Hazard Statements	H317 - H334
Precautions	P280 - P285
Reaction	P302 +P352 - P304 +P341 - P333 +P313 - P342 +P311

EINECS: 200-708-1 • WGK: 1 • HS: 29411000
Storage Temperature: +2 °C to +8 °C