Reagents and instruments for immunology, cell biology and molecular biology.

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

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Cat# E-BC-K009-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle	Under alkaline conditions, alkaline phosphatase catalyzes the hydrolysis of p-nitrobenzene phosphate disodium to produce p-nitrophenol and phosphoric acid. Under strong alkaline conditions, p-nitrophenol is bright yellow and has a maximum absorption peak at 405 nm. Therefore, the activity of ALP can be calculated by measuring the OD value at 405 nm.
Synonyms	ALP
Sample Type	Serum,Plasma,Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (400-415 nm, optimum wavelength: 405 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.27 U/L
Detection Range	0.27-50.8 U/L
Precision	inter-assay CV: 4.6% \| intra-assay CV: 1.2%
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K094-M	Acid Phosphatase (ACP) Activity Assay Kit	500Assays , 96T
E-BC-K196-M	5’-Nucleotidase (5'-NT) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	10-20
Mouse serum	20-30
Rat serum	10-20
Mouse plasma	10-20
HepG2 supernatant	8-12
10% Rat kidney tissue homogenate	400-1200
10% Mouse liver tissue homogenate	10-15
10% Rat lung tissue homogenate	50-100
10% Mouse brain tissue homogenate	30-50
Human urine	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.